多重耐药铜绿假单胞菌β-内酰胺酶检测与分析

目的了解本地区多重耐药铜绿假单胞菌β-内酰胺酶产生情况,为临床治疗及控制医院感染提供依据。方法用K-B法进行常规药敏试验,筛选出50株多重耐药铜绿假单胞菌,用改良三维试验检测各种β-内酰胺酶,同时用2-巯基丙酸抑制试验检测金属酶。结果50株多重耐药铜绿假单胞菌中产ESBLs2株(4%)、产AmpC酶20株(40%),11株同时产ESBLs和AmpC酶(22%),金属酶筛选出8株(16%)阳性。结论本地区多重耐药铜绿假单胞菌β-内酰胺酶以AmpC酶为主,临床应根据药敏试验结果,合理选用抗生素,以减少耐药菌株产生和控制医院感染。

Detection and analysis of beta-lactamases on multi-resistant Pseudomonas aeruginosa

Objective To study the production of beta-lactamases on multi-resistant Pseudomonas aeruginosa, and provide the guideline for treatment and control of Pseudomonas aeruginosa infection in hospital. Methods 50 strains of multi-resistant Pseudomonas aeruginosa were selected with K-B susceptibility method, The three-dimensional method was taken to differentiate the various beta-lactamases, 2-mercaptopropanoic acid inhibited detection was taken to metallo-beta-lactamases at the same time. Results Among 50 strains of multi-resistant Pseudomonas aeruginosa, there were 2 strains (4%) producing ESBLs, 20 strains(40%)producing AmpC beta-lactamases, and 11 strains(22%) producing ESBLs and AmpC beta-lactamases at the same time. 8 strains of them was producing metallo-beta-lactamases. Conclusions The main beta-lactamases was AmpC beta-lactamases in the multi-resistant Pseudomonas aeruginosa cultured in our area. In order to reduce the drug-resistance strains and control the infection of Pseudomonas aeruginosa, antibiotics should be used reasonably according to drug susceptibility test.