用显微分光光度计对几种不同类型肺癌癌细胞DNA含量的初步观测

本文用显微分光光度计对39例手术切除的不同组织学类型的人体肺癌标本癌细胞核的DNA含量进行了测量。其中高、中、低分化的鳞癌分别为3、6和5例;高、中、低分化的腺癌分别为3、5和5例;未分化癌大细胞型和小细胞型各为3例和6例。结果发现各类型肺癌的DNA含量均有统计学上的差异(P<0.01)。各DNA含量直方图的峰值部位及分布范围亦不同。从而提示了根据DNA含量的不同,可能有助于肺癌组织学类型及其分化程度的判别:而DNA含量直方图对肺癌类型的判别亦可能是有益的。

Determination of DNA content in Different Human Lung Cancerswith Microspectrophotometer

The DNA contents of 39 surgically resected specimens of different histopa-thological kinds and types of human lung cancer were determined with microspec-trophotometer. Among them, 3, 6, and 5 cases were diagnosed as well, moderately and poorly differentiated squamous cell carcinoma; 3,5, and 5 as well, moderately and poorly differentiated adenocarcinoma; and 3 and 6 cases as large and small cell types of undifferentiated carcinoma respectively.It was found that the DNA contents of different histopathological kinds and types of lung cancers were significantly different(P<0.01). The histograms of various kinds and types of lung cancers based on the values of DNA contents were also different.On the basis of this study, it is suggested to make more precise classification of human lung cancers according to the DNA contents, and the histograms of the DNA contents of lung cancers are helpful in the identification of the pathological kinds and types of the cancers.