茵陈五苓散对大鼠酒精性肝损伤防治作用的研究

目的观察茵陈五苓散对酒精性肝损伤大鼠血清ALT、AST和病理组织学的影响,为临床酒精性肝损伤的防治提供实验依据。方法将50只Wistar大鼠随机分成正常组、模型组、预防1组、预防2组、治疗组,采用白酒灌胃造模,于实验第5周、第9周末尾静脉取血,分离血清,测定ALT、AST。并于第9周取血后肝脏取材,常规HE染色,光镜观察。结果模型组血清ALT、AST明显升高,预防1组、预防2组、治疗组血清ALT、AST均明显低于模型组(P均<0.01)。预防组、治疗组肝组织病理学改变较模型组显著减轻。结论茵陈五苓散能有效预防和治疗大鼠酒精性肝损伤。

Study of Yinchen-Wuling powder on preventive and therapeutic effect of alcoholic liver injury in rats

Objective It is to observe the influence of Yinchen-Wuling powder on serum ALT,AST and pathohistology in rats with alcoholic liver injury,so to provide experiment evidence for clinic prevention and cure of the disease.Methods 50 Wistar rats were used to make models with filling the stomachs with alcohol and were randomly divided into normal group,model group,prevention group 1,prevention group 2 and treatment group.Serum from caudal vein was gotten and ALT,AST were measured after five and nine experiment weeks.Liver tissue was extracted and stained with HE to be observed with light microscope at the end of experiment.Results Serum ALT and AST in model group obviously increased which were significantly higher than those in prevention group 1,prevention group 2 and treatment group(P<0.01).The pathologic changes of liver tissue in prevention and treatment groups were obviously lighter than that in model group.Conclusion Yinchen-Wuling powder can effectively prevent and cure alcoholic liver injury of rats.