母乳与新生儿粪便中乳铁蛋白含量测定及相互关系的探讨

本文用火箭电泳法和酶联免疫吸附法分别测定了人奶和不同喂养方式新生儿粪便乳铁蛋白(LF)。结果显示,正常产妇1～8天初乳LF含量为6.88±3.10mg/ml,1～4个月成熟乳为3.18±0.87mg/ml。产后头3天初乳LF含量最高,之后渐下降,成熟乳阶段趋于稳定。不同喂养方式新生儿粪便LF含量有非常显著性差异(P<0.01)。粪便LF检出率,母乳喂养儿为100％,人工喂养儿仅60％。胎便中可检出小量,检出率为35％。母乳与新生儿粪便LF同步测定结果显示,母乳中高浓度LF对母乳喂养儿肠道LF含量有肯定的影响。人体肠道内源性LF的产生,从胎儿期就已开始,但在新生儿早期产生十分有限。母乳喂养能通过向新生儿传递包括LF在内的多种免疫物质,弥补生命早期自体产生的不足。

MEASUREMENT OF LACTOFERRIN IN HUMAN MILK AND NEONATAL FAECES AND EXPLORATION OF RELATION BETWEEN THEM

The concentrations of lactoferrin (LF) in colostrum during the first 8 days and mature milk during the first 4 months of postpartum were determined by electroimmunodiffusion. Meanwile a sensitive enzyme linked imm-unosorbent assay for measurements of LF in neonatal faeces were established. The results showed that human milk, especially colostrum, was rich in LF. The colostrum contained 6.88 ± 3.10mg/ml (M±SD) aad mature milk 3.18 ± 0.37mg/ml LF. LF in human milk was the highest during the first 3 days of lactation with a decline in concentration as lactation proceeds although a wide variation in concentration was noted among different individuals.LF in neonatal faeces showed significant difference in different feeding manners. LF level in the breast-fed infants was 19 times higher than that in the bottle-fed infants. The LF percentage detected in the former was 100% and the latter only 60%. The dynamic variations of LF in faeces of breast-fed infants during the first day of life were found to be relative to LF level in mother'milk.These data demonstrated the influence of high LF level in mother'milk on the concentrations of LF in breast-fed infants' intestine.Furthermore a few amounts of LF was found in meconium and its percentage detected was 35%. This suggested that the production of endogenous LF began from foetus period, but was limited during the first day of life. LF ingested by the breast-fed infants were not completely destroyed and thus may supplement the insufficiency of the endogenous LF in the newborn infants' intestinal tract.