卵母细胞浆内单精子显微注射治疗男性不育的效果

①目的 探讨卵母细胞浆内单精子显微注射(ICSI)技术治疗因严重少、弱精及阻塞性无精子症引起的男性不育的效果。②方法 对28对不育夫妻中的女方经控制性超促排卯技术获得卵子．男方少、弱精子症者采用梯度密度离心法分离精子，阻塞性无精子症者采用附睾或者睾丸穿刺获得精子，选择成熟卵子行ICSI。胚胎发育至4～8个细胞时移植。③结果 32个治疗周期中，获卵361个．其中成熟卯306个，ICSI后256个受精．受精率为83．7％。卵裂233个，卵裂率91％。临床妊娠11例，临床妊娠率34．4％。④结论 ICSI是治疗男性重症少、弱精子症引起的不育症的有效方法，

CLINICAL APPLICATION OF INTRACYTOPLASMIC SPERM INJECTION FOR MALE INFERTILITY CASES

Objective To apply the technique of intracytoplasmic sperm injection (ICSI) to male infertility resulting from severe oligozoospermia, asthenozoospermia and obstructive azoospemia. Methods After genetic counseling and chromsome ana- lysis, 28 couples were accepted. Occytes were collected from wives with controlled ovarian stimulation. Sperm from husbands with oligozoospermia or asthenozoospermia by density-gradient centrifugation, or from whom with obstructive azoospermia by epididymal sperm aspiration or testicular sperm aspiration . Mature oocytes were fertilized by ICSI. Results 361 oocytes were collected in 32 cycles. Among which 306 were mature. 256 were fertilized after ICSI. The fertilization rate was 83.7%. 233 oocytes developed to the stage of cleavage. The cleavage rate was 91%. Clinical pregnancy was achieved in 11 cycles. The pregnancy rate was 34.4%. Conclusion The technique of ICSI is an efficient method for the male infertility therapy.