再生障碍性贫血患者T淋巴细胞早期激活功能的研究

目的:了解再生障碍性贫血(AA)患者T淋巴细胞早期激活功能的变化。方法:应用流式细胞仪分析了34例AA患者骨髓和外周血单个核细胞T淋巴细胞亚群的变化以及CD4 和CD8 T细胞培养前后CD69的表达情况。结果:AA患者存在T细胞亚群数量的异常,CD4 /CD8 比值的下降与对照组比较有显著差异。重症AA患者体内有一部分CD4 与CD8 细胞表达CD69,而慢性AA患者只有部分CD8 细胞表达CD69,应用PHA刺激T细胞4 h后,AA和正常对照CD4 与CD8 T细胞CD69的表达率均迅速升高,但重症AA和慢性AA患者CD69的表达率均显著高于正常对照。结论:AA患者不仅存在T细胞数量异常,而且其T细胞早期激活功能也异常,可能与AA发病机制有关。

Early activation of T lymphocytes in patients with aplastic anemia

Objective:To investigate the early activafion of T lymphocytes in patients with aplastic anemia(AA).Methods: Expressions of CD4,CD8 T lymphocytes and CD69 in these lymphocytes before and after culture with PHA in vitro were analyzed by two-color flow cytometry in 34 patients with aplastic anemia.Results: There was significantly lower expression of CD4 /CD8 in aplastic anemia than normal controls.CD4 and CD8 lymphocytes partly expressed CD69 in severe AA patients,and only CD8 cells expressed CD69 in chronic AA.After PHA stimulation in vitro,expression rates of CD69 in CD4 and CD8 cells increased quickly in AA patients and controls,but significantly higher in AA patients than that in controls.Conclusion: In AA patients there are abnormalities not only in quantity of T cell subsets,but also in function of T cells early activation,which might be associated with the pathogenesis of AA.