中药红花注射液提高游离皮瓣存活率的研究

目的：应用中药红花注射液减轻皮瓣缺血再灌注损伤，从而提高游离皮瓣存活率。方法：首先建立新西兰大白兔游离皮瓣缺血动物模型，缺血时间控制在1．5h。实验分两组，实验组18只，在夹血管蒂前、松血管蒂后及术后治疗时静脉注射中药红花注射液；对照组18只注射生理盐水。观察皮瓣成活面积、皮瓣切片毛细血管生长情况，测定皮瓣组织中SOD、MDA含量并进行比较。结果：术后14d，实验组皮瓣平均存活面积（83．16±6．0）cm。显著高于对照组（55．28±7．5）cm。（P〈0．01）；实验组可见较多毛细血管生长，对照组仅在纤维组织中见少量毛细血管；实验组皮瓣组织在术后3d、7d、14d测定SOD活力（328．47±1．86，168．32±5．34，59．67±9．61，nmol／mgprotein）均大于对照组（144．75±1．58，85．21±8．56，15．29±6．48）（P〈0．01），提示实验组皮瓣中有较多的金属蛋白酶；实验组皮瓣组织在术后3d、7d、14d测定MDA含量（184．37±5．17，75．63±8．25，16．09±3．53，Nu／mgprotein）均小于对照组（242．61±7．24，175．23±8．36，22．18±4．64）（P〈0．01），间接反映对照组皮瓣自由基含量较多。结论：红花注射液对提高游离皮瓣存活率有明显的效果。

USE Safflower Injection of Chinese Herbal Medicine to Improve the Survival Rate of Free Flap

Objective： Use of traditional Chinese medicine to alleviate red skin injection ischemia-reperfusion injury, and improve the survival rate of free flap. Methods ： First of all, the establishment of New Zealand white rabbits free flap animal model of ischemia is made, ischemic time control in 1.5h. The rabbits divided into two groups： In experimental group,the Chinese medicine Safflower is intravenous injected before clip, after loose vessel pedicle, and after operations to 18 rabbits ; In the control group injected with normal saline to 18 rabbits. Area of survival flap, the growth of capillaries in flap sections observation, skin tissue SOD, MDA determination is analyzed through t-test. Results： 14d after the operation, the average flap tissue survival area of the experiment group （83. 16 ± 6.0 ） is obviously higher than control group （ 55.28±＋ 7.5 ） （ P 〈 0. 01 ）, there are many capillaries growed in flap tissue of experiment group, but just few capillaries growed in the Fibrous tissue of control group rabbits, the sod vitality of 3d ,7d, 14d in flap tissue of experiment group（ （328.47 ± 1.86,168.32 ± 5.34,59.67 ± 9.61 ） is higher than control group （ 144.75 ± 1.58,85.21 ± 8.56, 15.29± 6.48 ） （ P 〈 0.01 ）, pointed out that there is much metalloprotease in experiment group;the MDA value of flap tissue after 3d,7d, 14d（ 184.37 ± 5.17,75.63± 8.25,16.09± 3.53）is lower than control group（242.61 ±7.24,175.23 ± 8.36,22.18 ± 4.64） （ P 〈 0. 01 ）, pointed out indirectly there is much freeradical in the flap tissue of control group. Conclusion： safflower injection on the free flap has obvious effect on improvement of the survival flap tissue rate.