胃转流术对糖尿病大鼠降糖作用的实验研究

目的研究胃肠转流手术(GBP)对非肥胖型糖尿病大鼠的降糖效果与术后进食量、体重的关系。方法健康雄性wistar大鼠,腹腔注射链脲佐菌素(STZ)建立糖尿病模型。随机分成3组,O组:GBP手术组;F组:饮食控制组;C组:空白对照组。观察各组大鼠体重、平均进食量,分别测定术前、术后1、2、3、4、8周空腹及灌服葡萄糖30min后血糖水平。结果 O组GBP后4周,空腹和灌服葡萄糖后血糖分别由(20.8±4.9)、(30.2±3.1)mmol/L下降到(11.6±2.9)、(16.6±3.1)mmol/L(P<0.01)。O组体重改变无统计学意义。F组控制平均进食量约为O组的1/3并致体重显著下降,血糖下降没有O组明显。结论胃转流术对糖尿病大鼠具有明显治疗作用,其降糖作用与术后体重下降及进食量无关。

Effects of gastric bypass on blood glucose in rats with diabetes mellitus

Objective To investigate the effects of gastric bypass （GBP） on hypoglycemic activity,food-intake after GBP and body weight in rats with non-obesity diabetes mellitus.Methods The animal models with diabetes mellitus were established in 24 male Wistar rats by intraperitoneal injection with STZ,then the rats were randomly divided into 3 groups：group O （GBP）,group F （food restriction） and group C （blank controls）.The blood glucose concentrations were measured respectively at fasting and postprandial condition before and on the 1st,2nd,3rd,4th and 8th week after GBP for all the rats,and the rats＇ body weight and average food-intake were detected simultaneously.Results At the 4th week after GBP,the fasting and postprandial blood glucose levels in group O were significantly decreased from （20.8±4.9）,（30.2±3.1） mmol/L to （11.6±2.9）,（16.6±3.1） mmol/L,respectively （P0.01）.However there was no significant difference in the rats’ weight changes in group O.The average food-intake in group F was about one-third of group O,furthermore,the rats’ body weight was decreased,but the decrease of body weight was not as obvious as that in group O.Conclusion GBP has obvious therapeutic effect on diabetic rats,and its hypoglycemic activity is not related with the decrease of body weight after CBP and food-intake.