兰州地区回族与汉族儿童维生素D受体基因多态性分布

目的：了解兰州地区回族与汉族儿童维生素D受体(VDR)基因多态性分布,探讨不同种族之间VDR基因型频率的分布。方法：利用限制性内切酶Bsm Ⅰ,采用聚合酶链反应限制性片段长度多态性技术(PCR-RFLP),对兰州地区回族健康儿童81例和汉族健康儿童169例VDR基因多态性进行检测。结果：发现在回族81例中,bb型57例(70.4％),Bb型22例(27.2％),BB型2例(2.5％);在汉族169例中,bb型154例(91.1％),Bb型12例(7.1％),BB型3例(1.8％)。结论：兰州地区回族VDR基因多态性分布与汉族相比存在显著性差异。

Distribution of Vitamin D Receptor Gene Polymorphism in Hui and Han Ethnic Children in Lanzhou Region

Objective To investigate the distribution of vitamin D receptor (VDR) gene polymorphism in Hui and Han ethnic children in Lanzhou region and to study the difference between children of the two different ethnic groups. Methods VDR genotypes were assayed by polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) in 81 healthy Hui ethnic children and in 169 healthy Han ethnic children. Results Frequencies of bb, Bb and BB genotype were 70. 4% , 27. 2% and 2.5% respectively in the 81 Hui ethnic children and they were 91.1 % , 7.1% and 1.8% respectively in the 169 Han ethnic children. Conclusions There is a significant difference in the frequency distribution of VDR genotype between Hui and Han ethnic children. [Chin J Contemp Pediatr, 2003, 5(1): 37-38]