CEA特异性RNAi增强基因工程腺病毒H101治疗人食管癌裸鼠皮下移植瘤

目的 研究癌胚抗原（CEA）特异性基因沉默对基因工程腺病毒H101杀伤食管癌EC9706细胞作用的影响，以探讨影响H101敏感性的内在因素。方法 利用RNA干扰(RNAi) 技术，构建针对人食管癌EC9706细胞的CEA siRNA载体，通过基因转染, 在EC9706细胞中建立稳定的CEA基因沉默体系，并以空载体转染和未进行转染的EC9706细胞作对照，建立人食管癌细胞裸鼠皮下移植瘤模型, H101瘤内注射。用RT-PCR和免疫组化法检测CEA在移植瘤中的表达,测量肿瘤体积。结果 降低CEA在mRNA和蛋白质水平的表达，对人食管癌EC9706细胞裸鼠皮下移植瘤成瘤时间和大小无明显影响，H101瘤内注射后，干扰组肿瘤体积显著低于空载体组和对照组（P＜0.05）。结论 抑制食管癌EC9706细胞中CEA的表达, 能增强H101的敏感性。

Enhancement of CEA specific RNAi on gene engineering adenovirus H101 for treatment of tumor tissue of nude mice transplanted with human esophageal cancer cells

Objective To study the effect of gene engineering adenovirus H101 for treating esophagus carcinoma EC9706 induced by CEA gene specific silencing and to explore internal influential factor of H101 sensitivity. Methods To construct the siRNA expression vector of CEA and to inhibit the expression of CEA through RNA interference and gene transfection in EC9706 cell,stable CEA gene silencing system was set up,compared with empty vector group and non-transfected EC9706 cell,the model of athymic mouse subcutaneous...