| 慢病毒载体法制备转基因动物研究进展 |
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| 引用本文： | 黄黎珍,刘光泽,顾为望.慢病毒载体法制备转基因动物研究进展[J].实验动物与比较医学,2007,27(1):63-66. |
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| 作者姓名： | 黄黎珍 刘光泽 顾为望 |
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| 作者单位： | 1. 南方医科大学实验动物中心,广州,510515
2. 中国人民解放军第458医院肝病重点实验室,广州,510600 |
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| 摘 要： | 慢病毒为逆转录病毒的一种,除了具有逆转录病毒的基本特性外,还具有感染静止细胞的特性。最近研究发现,应用慢病毒载体进行转基因动物制备,转基因的效率得到显著提高,使转基因动物研究领域产生突破性进展。本文介绍了慢病毒载体构建的基本原理、慢病毒载体制备转基因动物的常见方法及国内外研究的最新进展。
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| 关 键 词： | 慢病毒载体 逆转录病毒 转基因动物 |
| 文章编号： | 1004-8448(2007)01-0063-04 |
| 修稿时间： | 2006-09-08 |
Review on Lentivirus Transgenesis for Producing Transgenic Animal |
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| HUANG Li-zhen,LIU Guang-ze,GU Wei-wang.Review on Lentivirus Transgenesis for Producing Transgenic Animal[J].Laboratory Animal and Comparative Medicine,2007,27(1):63-66. |
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| Authors: | HUANG Li-zhen LIU Guang-ze GU Wei-wang |
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| Institution: | 1. Laboratory Animal Centre of Southern Medical University, Guangzhou 510515, China; 2. Infections Disease Centre of PLA, 458^th Hospital of PLA, Guangzhou 510602, China |
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| Abstract: | Lentiviruses belong to the retroviruse and they are characterized by infecting non-dividing cells except for sharing many characteristic features with simple retroviruses. The recent studies found that the transgenic efficiency was enhanced significantly with lentiviral transgenesis. Due to its high efficiency, this method will make a breakthrough in transgenic animal research field. This article is about the principle of constructing lentiviral vector, the common approach of producing transgenic animal with lentiviral transgenesis and its latest progress home and abroad. |
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| Keywords: | Lentiviral vector Retrovirus Transgenic animal |
| 本文献已被 CNKI 维普 万方数据 等数据库收录！ |
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