血管紧张素转换酶、内皮型一氧化氮合酶和载脂蛋白E基因多态性与冠心病的相关性

 目的]研究血管紧张素转换酶（ACE）基因插入/缺失多态性、内皮型一氧化氮合酶（eNOS）基因G894T多态性和载脂蛋白E（ApoE）基因多态性与冠状动脉粥样硬化性心脏病（冠心病）的关系.方法]应用基因芯片技术分析133例冠心病患者和122例对照者ACE、eNOS和ApoE基因多态性,对比两组基因型及等位基因频率.结果]冠心病组ACE DD基因型频率比对照组显著升高,28.6% vs 13.1%,P＜0.01,ACE基因多态性与冠心病危险性相关.两组eNOS和ApoE基因型频率差异无统计学意义（P＞0.05）,eNOS和ApoE基因多态性与冠心病危险性无明显相关.结论]ACE基因多态性可能是中国人冠心病的危险因素,基因芯片技术可能是研究多种易感基因与冠心病相关性的一种高效、敏感的方法.

Relationship of Angiotensin-converting Enzyme, Endothelial Nitric Oxide Synthase and Apolipoprotein E Gene Polymorphisms with Coronary Disease

Objective] To study angiotensin-converting enzyme (ACE) gene insertion/deletion polymorphism, endothelial nitric oxide synthase (eNOS) G894T polymorphism and apolipoprotein E(ApoE) gene polymorphism in relation to coronary heart disease(CHD). Methods] ACE, eNOS, and ApoE gene polymorphisms of 133 patients with CHD and 122 controls were analyzed by gene chip technology. The allele frequency and genotype distribution were compared between the two groups. Results] The frequency of DD genotype of ACE gene in patients with CHD was higher than that of control(28.6% vs 13.1%, P < 0.01). The ACE gene polymorphism was associated with an increased risk of CHD. The difference of eNOS and ApoE genotype distribution between the two groups was not significant (P >0.05). The eNOS and ApoE gene polymorphisms were not associated with an increased risk of CHD. Conclusion] The ACE gene polymorphism may be a risk factor of CHD in Chinese. Gene chip technology may be an effective and sensitive method to study the relationship of gene polymorphisms and CHD.