Biological responses of silver-coated thermosets: An in vitro and in vivo study |
| |
Authors: | E. Marsich A. Travan I. Donati G. Turco J. Kulkova N. Moritz H.T. Aro M. Crosera S. Paoletti |
| |
Affiliation: | 1. Department of Life Sciences, University of Trieste, Via Giorgieri 5, Trieste I-34127, Italy;2. Division of Dental Sciences and Biomaterials, Department of Medical, Surgical and Health Sciences, University of Trieste, Piazza dell’Ospitale 1, Trieste I-34129, Italy;3. Orthopaedic Research Unit, Department of Orthopaedic Surgery and Traumatology, University of Turku and University Hospital of Turku, 20520 Turku, Finland;4. Turku Clinical Biomaterials Centre (TCBC), Institute of Dentistry, University of Turku, Itäinen Pitkäkatu 4B, 20520 Turku, Finland;5. Department of Chemical and Pharmaceutical Sciences, University of Trieste, Via Giorgieri 1, Trieste I-34127, Italy |
| |
Abstract: | Bisphenol A glycidylmethacrylate (BisGMA)/triethyleneglycol dimethacrylate (TEGDMA) thermosets are biomaterials commonly employed for orthopedic and dental applications; for both these fields, bacterial adhesion to the surface of the implant represents a major issue for the outcome of the surgical procedures. In this study, the antimicrobial properties of a nanocomposite coating formed by polysaccharide 1-deoxylactit-1-yl chitosan (Chitlac) and silver nanoparticles (nAg) on methacrylate thermosets were studied. The Chitlac–nAg system showed good anti-bacterial and anti-biofilm activity although its biocidal properties can be moderately, albeit significantly, inhibited by serum proteins. In vitro studies on the silver release kinetic in physiological conditions showed a steady metal release associated with a gradual loss of antimicrobial activity. However, after 3 weeks there was still effective protection against bacterial colonization which could be accounted for by the residual silver. This time-span could be considered adequate to confer short-term protection from early peri-implant infections. Preliminary in vivo tests in a mini-pig animal model showed good biological compatibility of Chitlac–nAg-coated materials when implanted in bony tissue. The comparison was made with implants of titanium Ti6Al4V alloy and with a Chitlac-coated thermoset. Bone healing patterns and biocompatibility parameters observed for nAg-treated material were comparable with those observed for control implants. |
| |
Keywords: | |
本文献已被 ScienceDirect 等数据库收录! |
|