Human corneal endothelial cell sheets for transplantation: Thermo-responsive cell culture carriers to meet cell-specific requirements |
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Authors: | J Teichmann M Valtink S Gramm M Nitschke C Werner RHW Funk K Engelmann |
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Institution: | 1. Institute of Anatomy, Medical Faculty Carl Gustav Carus, Technische Universität Dresden, Fetscherstrasse 74, 01307 Dresden, Germany;2. Leibniz Institute of Polymer Research Dresden, Max Bergmann Center of Biomaterials, Hohe Strasse 6, 01069 Dresden, Germany;3. CRTD/DFG-Center for Regenerative Therapies Dresden – Cluster of Excellence, Tatzberg 47/49, 01307 Dresden, Germany;4. Department of Ophthalmology, Klinikum Chemnitz gGmbH, Flemmingstrasse 2, 09116 Chemnitz, Germany |
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Abstract: | Corneal endothelial diseases lead to severe vision impairment, motivating the transplantation of donor corneae or corneal endothelial lamellae, which is, however, impeded by endothelial cell loss during processing. Therefore, one prioritized aim in corneal tissue engineering is the generation of transplantable human corneal endothelial cell (HCEC) layers. Thermo-responsive cell culture carriers are widely used for non-enzymatic harvest of cell sheets. The current study presents a novel thermo-responsive carrier based on simultaneous electron beam immobilization and cross-linking of poly(vinyl methyl ether) (PVME) on polymeric surfaces, which allows one to adjust layer thickness, stiffness, switching amplitude and functionalization with bioactive molecules to meet cell type specific requirements. The efficacy of this approach for HCEC, which require elaborate cell culture conditions and are strongly adherent to the substratum, is demonstrated. The developed method may pave the way to tissue engineering of corneal endothelium and significantly improve therapeutic options. |
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