Mechanism of the uptake of cationic and anionic calcium phosphate nanoparticles by cells |
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Authors: | Viktoriya Sokolova Diana Kozlova Torben Knuschke Jan Buer Astrid M Westendorf Matthias Epple |
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Institution: | 1. Inorganic Chemistry and Center for Nanointegration Duisburg-Essen (CeNIDE), University of Duisburg-Essen, Universitaets str. 5-7, D-45117 Essen, Germany;2. Medical Microbiology, University Hospital Essen, University of Duisburg-Essen, Hufeland str. 55, 45147 Essen, Germany |
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Abstract: | The uptake of calcium phosphate nanoparticles (diameter 120 nm) with different charge by HeLa cells was studied by flow cytometry. The amount of uptaken nanoparticles increased with increasing concentration of nanoparticles in the cell culture medium. Several inhibitors of endocytosis and macropinocytosis were applied to elucidate the uptake mechanism of nanoparticles into HeLa cells: wortmannin, LY294002, nocodazole, chlorpromazine and nystatin. Wortmannin and LY294002 strongly reduced the uptake of anionic nanoparticles, which indicates macropinocytosis as uptake mechanism. For cationic nanoparticles, the uptake was reduced to a lesser extent, indicating a different uptake mechanism. The localization of nanoparticles inside the cells was investigated by conjugating them with the pH-sensitive dye SNARF-1. The nanoparticles were localized in lysosomes after 3 h of incubation. |
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