Loop-Mediated Isothermal Amplification Test for Detection of Neisseria gonorrhoeae in Urine Samples and Tolerance of the Assay to the Presence of Urea |
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| Authors: | Thomas Edwards Patricia A. Burke Helen B. Smalley Liz Gillies Glyn Hobbs |
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| Affiliation: | aSchool of Pharmacy and Biomolecular Sciences, Liverpool John Moores University, Liverpool, United Kingdom;bMast Group Ltd., Bootle, Liverpool, United Kingdom |
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| Abstract: | A loop-mediated isothermal amplification (LAMP) assay for open reading frame 1 (ORF1) of the glutamine synthetase gene of Neisseria gonorrhoeae was able to tolerate urea concentrations of ≤1.8 M, compared with a PCR assay that was functional at concentrations of <100 mM. The LAMP assay was as sensitive as the PCR assay while being faster and simpler to perform. |
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