Simple,Low-Cost Molecular Assays for TR34/L98H Mutations in the cyp51A Gene for Rapid Detection of Triazole-Resistant Aspergillus fumigatus Isolates |
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| Authors: | Suhail Ahmad Ziauddin Khan Ferry Hagen Jacques F. Meis |
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| Affiliation: | aDepartment of Microbiology, Faculty of Medicine, Kuwait University, Kuwait City, Kuwait;bDepartment of Medical Microbiology and Infectious Diseases, Canisius Wilhelmina Hospital, Nijmegen, The Netherlands;cDepartment of Medical Microbiology, Radboud University Medical Center, Nijmegen, The Netherlands |
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| Abstract: | Simple, low-cost PCR/PCR-restriction fragment length polymorphism (RFLP) assays targeting cyp51A promoter and codon 98 regions were developed for the detection of triazole-resistant Aspergillus fumigatus strains carrying TR34/L98H mutations. The assays were evaluated using 40 itraconazole-susceptible isolates and 35 itraconazole-resistant isolates. The prevalence of TR34/L98H mutations in clinical/environmental A. fumigatus isolates may now be determined easily from resource-poor settings. |
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