| MiRNA-27a对3T3-L1细胞胰岛素抵抗的作用及机制简 |
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| 引用本文： | 谢军,蔡克银.MiRNA-27a对3T3-L1细胞胰岛素抵抗的作用及机制简[J].华南国防医学杂志,2014(2):97-99,112. |
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| 作者姓名： | 谢军 蔡克银 |
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| 作者单位： | 中国人民解放军广州军区武汉总医院干部病房二科,湖北武汉430070 |
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| 摘 要： | 目的研究miRNA-27a对3T3-L1细胞葡萄糖摄取的影响，并初步探讨其作用机制。方法通过肿瘤坏死因子α（tumornecrosisfactoralpha，TNF-α）诱导建立3T3-L1脂肪细胞胰岛素抵抗的细胞模型，观察miRNA-27a对3T3-L1细胞葡萄糖摄取的影响，酶联免疫吸附（enzyme-linkedimmunosorbentassay，ELlSA）检测细胞培养上清中白细胞介素6（interleukin6，IL-6）的变化，Westernblot检测细胞内总Akt和磷酸化Akt的变化。结果miRNA-27a明显抑制3T3-L1细胞对胰岛素诱导的葡萄糖的摄取，miRNA-27a可促进炎症因子IL-6的产生并抑制Akt的磷酸化。结论miRNA-27a可促进脂肪细胞产生胰岛素抵抗，其作用可能通过抑制胰岛素信号通路P13K／Akt实现。
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| 关 键 词： | miRNA-27a 胰岛素抵抗3T3-L1脂肪细胞 PBK Akt信号通路 |
Effect and Mechanism of MiRNA-27a on Insulin Resistance in 3T3-L1 Cells |
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| XIE J un,CAI Ke-yin.Effect and Mechanism of MiRNA-27a on Insulin Resistance in 3T3-L1 Cells[J].Military Medical Journal of South China,2014(2):97-99,112. |
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| Authors: | XIE J un CAI Ke-yin |
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| Institution: | . (The Second Cadre Ward, Wuhan General Hospital of Cruangzhou Command, Wuhan Hubei 430070, China) |
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| Abstract: | Objective To research the influence of miRNA-27a on glucose uptake of 3T3-L1 adipocyte and the probability mechanisms. Methods 3T3-L1 cell model with insulin resistance was established by tumor necrosis factor al- pha （TNF-α） induction. The influence of miRNA-27a on glucose uptake of 3T3-L1 adipocyte was observed. Changes of in- terleukin 6 （IL-6） levels were tested by enzyme-linked immunosorbent assay （ELISA）. Changes of total Akt and Akt phosphorylation were tested by Western blot. Results MiRNA-27a significantly inhibited the glucose uptake of 3T3-L1 adipocyte,and promoted IL-6 secretion and inhibited phosphorylation of Akt. Conclusion miRNA-27a can promote insulin resistance of adipocyte possibly by inhibiting PI3K/Akt signal pathway. |
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| Keywords: | MiRNA-27a Insulin resistance 3T3-L1 adipocyte PI3K/Akt signal pathway |
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