| 电离辐射对血管内皮细胞影响的实验研究 |
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| 引用本文： | 张志琳,霍红梅,王玉祥,田野,包仕尧.电离辐射对血管内皮细胞影响的实验研究[J].苏州大学学报(自然科学版),2002,22(4):383-385. |
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| 作者姓名： | 张志琳 霍红梅 王玉祥 田野 包仕尧 |
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| 作者单位： | 苏州大学附属第二医院 苏州215004
(张志琳，霍红梅，王玉祥，田野)，苏州大学附属第二医院 苏州215004(包仕尧) |
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| 摘 要： | 目的 观察电离辐射 (X线 )对血管内皮细胞培养上清液中内皮素 (ET)和一氧化氮 (NO)含量变化及细胞凋亡的影响。方法 采用新生儿脐带静脉血管内皮细胞 (HUVEC)体外原代培养方法 ,用放免法和化学法检测培养上清液中ET和NO含量 ,用透射电镜、流式细胞仪观察细胞形态特征和检测凋亡细胞。结果 血管内皮细胞在电离辐射 2 4h后 ,各辐射剂量组培养上清液中NO含量无变化 ;在 2Gy剂量组ET含量变化不明显 ,而 5、10、2 0Gy各剂量组ET含量明显下降 (P <0 .0 5 )。电离辐射 2 4、4 8h后HUVEC形态无明显改变 ,未检测出明显凋亡细胞。结论 原代培养生长的HUVEC在 2～ 2 0Gy照射时有一定的放射抗性 ,其确切机理有待进一步研究
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| 关 键 词： | 实验研究 电离辐射 血管内皮细胞 内皮素 一氧化氮 细胞凋亡 |
| 文章编号： | 1000-5749(2002)04-0383-03 |
| 修稿时间： | 2002年3月22日 |
Experimental Research of Effects of Radiation on Vascular Endothelial |
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| ZHANG Zhi-lin,Huo Hong-mei,Wang Yu-xiang,et al..Experimental Research of Effects of Radiation on Vascular Endothelial[J].Suzhou University Journal of Medical Science,2002,22(4):383-385. |
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| Authors: | ZHANG Zhi-lin Huo Hong-mei Wang Yu-xiang |
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| Abstract: | objective To observe effect of radiation on contents of nitric oxide(No) and endothlin(ET) in culture solution of primary HUVECs and its effect on apoptosis.Methods HUVECs was primary culture,contents of ET-1 and NO were determined by radioimmunoassay and HNO 3 reduction method and detected apoptosis of radiated HUVECs was detected with transmission electron microscope(TEM)and flow cytometry(FCM).Result We found NO contents were not changed 24 hours after radiation. There was no unchang in ET contents after being radiated at a dose of 2Gy and there was reduction after radiation at a dose of 5-20Gy ( P <0.05).TEM and FCM failed to be detected increasing in apoptosis.Conclusion The culture primary HUVECs partly resist to radiation,its mechanism should be further researched. |
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| Keywords: | Radiation ?Vascular Endothelia cell ?Endothelin(ET) ?Nitric oxide(NO) ?Apoptosis |
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