Membrane-seeded autologous chondrocytes: cell viability and characterization at surgery |
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Authors: | Antonio Gigante Claudia Bevilacqua Andrea Ricevuto Monica Mattioli-Belmonte Francesco Greco |
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Affiliation: | (1) Department of Orthopaedics, Polytechnic University of Marche, Via Tronto 10, 60020 Ancona, Italy;(2) Institute of Normal Human Morphology, Polytechnic University of Marche, Ancona, Italy |
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Abstract: | The implantation of chondrocytes, seeded on matrices such as hyaluronic acid or collagen membranes, is a method that is being widely used for the treatment of chondral defects. The aim of the present study was to evaluate the distribution, viability and phenotype expression of the cells seeded on a collagen membrane just at the time of the implantation. Twelve patients who were suffering from articular cartilage lesions were treated by the MACI® procedure. The residual part of each membrane was tested by colorimetric assay (MTT) and histochemical and ultrastructural analyses were carried out. In all of the samples a large number of viable cells, quite homogenously distributed, was detected. The cells expressed the markers of the differentiated hyaline chondrocytes. These data reassure in that the MACI procedure provides a suitable engineered tissue for cartilage repair, in line with the clinical evidences emerging in the literature. |
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Keywords: | Chondrocytes Articular cartilage Cells Cultured Histocytochemistry Tissue engineering |
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