对苯二甲酸诱导大鼠泌尿系结石的成分分析

为研究对苯二甲酸（TPA）诱导大鼠泌尿系结石的成分，并为TPA诱导大鼠膀胱癌的可能机理提供线索，用喂饲法对大鼠连续染毒90天，收集膀胱和肾脏结石，用电感耦合等离子体发射光谱仪，元素分析仪对结石进行元素构成分析，用傅里叶变换红外光谱仪分析结石的有机物构成，结果提示，结石主要由碳，氢，氮，钙，磷等元素构成，红外光谱显示两种结石中均可能含有发生对位取代的苯环化合物，亚硝基化合物以及铵盐，碳酸盐等，但无TPA原形的存,在,提示TPA在体内经过了代谢，至少不完全以原形排出体外。

Analysis on the components of rat urinary stone induced by terephthalic acid

In order to analyze the components of rat urinary stone and to explore the possible mechanism of chemically induced bladder cancer, terephthalic acid (TPA) is orally administrated to rats at the doses of 5000 and 500 mg/kg/BW/everyday for consecutive 90 days and stones in bladder and kidney are collected. Inductive Coupled Plasma Quantomer (ICP), Element Analyzer (EA) and Fourier Transform Infared Spectrometer (FT-IR) are applied for the analysis on the components of stone in bladder and kidney. The results showed that the main components of the stone are calcium, carbon, nitrogen, hydrogen and phosphorus. The FT-IR showed that the stones both in bladder and kidney might contain nitrous, carbonate, ammonium salt, and para position replaced benzene ring compounds, but no original TPA is detected. The findings indicate that TPA might have a metabolic turnover inside the rat body, and, at least, might not be completely excreted in its original form.