口腔癌前病变和鳞癌组织中hMSH2蛋白表达的初步探讨

目的:探讨错配修复基因hMSH2的表达与口腔鳞癌发生、发展的关系.方法:采用Western Blot和免疫组化方法检测正常口腔上皮组织、癌旁组织和鳞癌组织中hMSH2的蛋白含量和组织分布.结果:Western Blot检测发现口腔正常上皮组织与癌旁上皮组织的hMSH2表达无明显差异(P＞0.05),而鳞癌上皮内hMSH2蛋白表达较前两者显著增加(P＜0.05).免疫组化结果显示正常上皮内仅见基底层细胞胞核阳性表达;癌旁上皮内hMSH2蛋白表达与异常增生程度有关,异常增生明显的上皮,胞核阳性表达的细胞亦较多;hMSH2蛋白在鳞癌上皮全层细胞胞核内呈阳性表达.所有细胞的胞浆均未见阳性表达.结论:在口腔鳞癌组织中hMSH2蛋白表达水平升高,表明hMSH2的表达在口腔鳞癌的发生、发展过程中可能存在表观遗传调节.

The Study of the Protein Expression of hMSH2 in the Tissues of Oral Premalignant Lesion and OSCC

Objective: To study the protein expression of mismatch repair (MMR) gene hMSH2 in the development of oral squamous cell carcinoma(OSCC). Methods: A total of 33 samples, including OSCC and corresponding tumor-adjacent and normal tissues, were investigated for the changes on the amount and location of hMSH2 protein in the development of OSCC by using Western Blot and immunohistochemical test. Results: Significantly higher expression of hMSH2 was detected in the samples of carcinoma, but no such promotion was found in the tumor-adjacent and normal tissues. The positive expression of hMSH2 protein in nuclei was only observed in the base cells of the normal tissue, while it was correlated to the development of dysplasia in the tumor-adjacent tissues. In the cancer tissues all the epithelial cells were detected to be positive immunoreactivily in nuclei. The cytoplasms of all the cells were shown negative immunoreactivity. Conclusion: There may be protective epigenetic inheritance regulation of hMSH2 in the carcinogenesis of OSCC.