| 当归对大鼠脑缺血半暗带细胞凋亡的抑制作用 |
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| 引用本文： | 章军建,张晓琴,黄建英. 当归对大鼠脑缺血半暗带细胞凋亡的抑制作用[J]. 卒中与神经疾病, 2001, 8(1): 8-10 |
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| 作者姓名： | 章军建 张晓琴 黄建英 |
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| 作者单位： | 1. 武汉，湖北医科大学附属第二医院神经科 430071
2. 武汉，湖北医科大学附属第二医院神经科科研处 430071 |
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| 基金项目： | 湖北省科委攻关课题(972P1215)和湖北省教委科研基金课题(99B009)资助。 |
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| 摘 要： | 目的 研究当归对脑缺血半暗带细胞凋亡的影响。方法 采用大鼠局灶性脑缺血再灌流模型，大脑中动脉阻塞2小时，再灌流48小时，用TUNEL法和免疫组织化学染色法分别检测使用当归后缺血半暗带凋亡细胞和凋亡相关基因Bcl-2、Bax蛋白表达。结果 与对照组比较，当归治疗组缺血半暗带的凋亡细胞显著减少（P＜0.01）；Bcl-2的表达显著增加（P＜0.01),Bax的表达无显著变化（P＞0.05）。结论 当归可能促进Bcl－2在脑缺血后的表达对半暗带的细胞凋亡产生抑制作用。
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| 关 键 词： | 脑缺血半暗带 当归 细胞凋亡 中医药疗法 |
| 文章编号： | 1007-0478(2001)01-0008-03 |
The apoptosis prevention of Angelica sinensis in ischemic penumbra following middle cerebral artery occlusion in the rat |
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| Zhang Junjian,Huang Jianying,Zhang Xiaoqing. The apoptosis prevention of Angelica sinensis in ischemic penumbra following middle cerebral artery occlusion in the rat[J]. Stroke and Nervous Diseases, 2001, 8(1): 8-10 |
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| Authors: | Zhang Junjian Huang Jianying Zhang Xiaoqing |
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| Affiliation: | Zhang Junjian,Huang Jianying,Zhang Xiaoqing.The Second Affiliated Hospital of Hubei Medical University,Wuhan 430071 |
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| Abstract: | Objective To investigate the effects of Angelica sinensis on apoptosis in ischemic penumbra following transient cerebral ischemia.Methods The middle cerebral arteries(MCA) of Wistar rats were occluded for 2 hours.The Angelica sinensis was administered intracavitary at 12g/kg.Apoptosis was detected by terminal deoxynucleotidyl transferase-mediated dUTP nick end-labeling(TUNEL).Bcl-2 and Bax expression was detected by immunohistochemistry method.Results In the Angelica sinensis group,the apoptotic cells were significantly decreased( P <0.01)and Bcl-2 protein expression was significantly increased( P <0.01) in ischemic penumbra than those mthe control group.Conclusion Angelica sinensis can prevent apoptosis in penumbra following cerebral ischemia due to the enhanced expression of Bcl-2. |
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| Keywords: | ischemic penumbra Angelica sinensis apoptosis |
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