| 肝硬化大鼠肾脏水通道蛋白2和血管加压素V2受体的表达及黄芪对其的作用 |
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| 引用本文： | 朱春晓,顾勇,陈靖,马骥,杨海春,林善锬. 肝硬化大鼠肾脏水通道蛋白2和血管加压素V2受体的表达及黄芪对其的作用[J]. 中华肾脏病杂志, 2002, 18(1): 45-48 |
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| 作者姓名： | 朱春晓 顾勇 陈靖 马骥 杨海春 林善锬 |
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| 作者单位： | 200040,上海,复旦大学附属华山医院肾内科 |
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| 基金项目： | 上海市卫生局百人计划资助项目（98BR038） |
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| 摘 要： | 目的 观察肝硬化大鼠肾脏水通道蛋白2（AQP2）和血管加压素（AVP）-V2受体的表达情况，及中药黄芪对其的作用。方法 肾脏皮髓制裁AQP2mRNA检测采用半定量RT-PCR法，肾脏AQP2和AVP-V2受体蛋白检测采用免疫组织化学法。结果 肝硬化2周和5周大鼠肾脏皮髓质AQP2mRNA，肾脏AQP2和AVP-V2受体蛋白表达上调，黄芪在2周时可纠正这些指标的升高，5周时则不能。结论 肝硬化大鼠存在AVP系统和AQP2表达的异常。黄芪在早期对其有改善作用。
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| 关 键 词： | 肝硬化 精氨酸升压素 水孔蛋白类 黄芪 血管加压素 |
| 修稿时间： | 2001-05-12 |
Expression of renal aquaporin-2 and AVP-V2 receptor in liver cirrhosis and the role of astragalus memberanaceus |
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| ZHU Chunxiao,GU Yong,CHEN Jing. et al. Expression of renal aquaporin-2 and AVP-V2 receptor in liver cirrhosis and the role of astragalus memberanaceus[J]. Chinese Journal of Nephrology, 2002, 18(1): 45-48 |
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| Authors: | ZHU Chunxiao GU Yong CHEN Jing. et al |
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| Affiliation: | ZHU Chunxiao,GU Yong,CHEN Jing. et al. Department of Nephrology,Huashan Hospital,Fudan University Shanghai 200040,China |
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| Abstract: | Objective To investigate the changes of AVP system and AQP2 in liver cirrhosis and the role of astragalus memberanaceus( AM) Methods Immunohistochemistry and relatively quantitative RT-PCR technique were used to determine the renal protein levels of AVP-V2 receptor and AQP2. and the renal levels of AQP2 mRNA of hepato cirrhotic rats induced by common bile duct ligation for 2 and 5 weeks. Results The hepato cirrhotic rats demonstrated obviously enhanced expression of AQP2 mRNA and protein, and V2 receptor protein in kidney. AM could alleviate the over expression of these parameters in compensated cirrhosis (2 weeks), but could not in decompensated cirrhosis (5 weeks) Condusion The abnormality of AVP system and AQP2 exists in hepato cirrhosis, which can be improved by AM in early stage. |
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| Keywords: | Liver cirrhosis Arginine vasopressin Aquaporins Astragalus memberanaceus |
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