HP感染蒙古沙土鼠致Barrett食管及胃癌的实验研究

目的 通过建立幽门螺杆菌(HP)感染蒙古沙土鼠的动物模型,观察HP及HP与N-甲基-N′-硝基-N-亚甲基胍(MNNG)共同作用后食管和胃黏膜的组织学改变.方法 96只SPF级蒙古沙土鼠随机分为4组,每组24只.A组单用HP菌液灌胃;B组在接种HP后4周,摄入MNNG水(20μg/ml),连续30周;C组单用MNNG水(20μg/ml),连续30周;D组为对照组.各组分别在实验后12、24和48周3个时相点各处死8只,取食管和胃黏膜行组织学检查,用Warthin-Starry银染、PCR和快速尿素酶法检测HP.结果 累计至48周,萎缩、肠化生和异型增生的发生率A组(62.5%,33.3%,37.5%)、B组(62.5%,20.8%,54.2%)显著高于C组(37.5%,4.2%,8.3%)、D组(0),差异有统计学意义(P＜0.01).A组有1例发生胃癌,2例出现Barrett食管.结论 HP感染可以直接导致胃癌发生,同时也能诱发出Barrett食管.

Study of Barrett esophagus and gastric carcinoma in Mongolian gerbils induccd by Helicobacter pylori infection

Objective Using a model of Helicobacter pylori(HP) infected Mongolian gerbil,to observe the effect of HP and N-methy-N'-nitro-N-nitrosoguanidine(MNNG) on gastric and esophagus mucosa.Methods A total of 96 Mongolian gerbils,six-week-old,were randomly divided into four groups(each 24 animals):group A infected with HP;group B infected with HP followed by MNNG administration;group C received MNNG without HP infection;group D as control.Eight animals from each group were killed at 12,24,48 weeks,and histopathological changes in their stomach and esophagus were examined for chronic gastritis,intestinal metaplasia,atypical hyperplasia,adenoma and Barrett esophagus.Results The incidences of intestinal metaplasia and dysplasia in group A and B were significantly higher than those in the other groups(P<0.01).Conclusion HP could directly induce gastric carcinoma and Barrett esophagus.