FK506对肝脏保存再灌注中肝窦内皮细胞损伤的保护作用

目的 探讨他克莫司（FK506）对肝脏低温保存再灌注中肝窦内皮细胞损伤的保护作用。方法 应用离体鼠肝脏保存再灌注模型，测定保存不同的时间后肝脏灌注流出液中内皮素（ET）及丙氨酸转氨酶（ALT）的含量以及透明质酸（HA）的摄取量，光镜及电镜下观察肝窦内皮细胞的形态学改变，比较分析FK506对上述指标的影响。结果 对照组随肝脏保存再灌注时间延长，灌注流出液中ET含量明显升高（P＜0．05），HA摄取明显降低（P＜0．05），并伴随ALT活性显著增高（P＜0．05）和肝窦内皮细胞形态学的异常改变；保存液和灌注液中均加入FK506后，上述指标异常变化均明显减轻，其差异有显著性（P＜0．05）。结论 FK506对离体大鼠肝脏肝窦内皮细胞的再灌注损伤有保护作用。

Preventive effect of tacrolimus (FK506) on sinusoidal endothelial cell injury du ring hepatic preservation and reperfusion

Objective　To investigate the structural and functional changes in sinusoidal endothelial c ells (SEC) during hepatic preservation and reperfusion and protective effect of tacrolimus (FK506). Methods　The changes in endothelin (ET) content, hyaluroni c acid (HA) uptake, alanine aminotransferase (ALT) activity and morphological ch anges in hepotocytes and SEC under light microscopy and electron microscopy were observed by using isolaec perfused rat liver model. The effect of FK506 on the above parameters were also observed. Results　In the control group with prolongation of liver preservation and reperfusion, E T content and ALT activity were increased remarkably (both P＜0.05), and abnomal morphological changes of hepatocytes and SEC were obvious. With the tre atment of FK506, the abnormal changes of all the above parameters were improved remarkably (P＜0.05). Conclusions　SEC injury may be the main causes of microcir culation disturbance and graft failure following reperfusion. FK506 could prev ent the isolated SEC from injury during hepatic preservation and reperfusion.