颈椎间盘组织化学增龄变化和病理改变

目的：研究颈椎间盘随年龄增长而产生的组织化学增龄变化和病理改变。方法：利用组织化学方法，观察胎儿、婴幼儿、成人和颈椎病患者的碱性和酸性磷酸酶以及过碘酸希夫氏反应（ｐｅｒｉｏｄｃａｃｉｄ－ｓｃｈｉｆｒｅａｔｉｏｎ，ＰＡＳ反应）。结果：患者组酸性磷酸酶呈阳性反应，胎儿、婴幼儿为阴性反应；碱性磷酸酶患者组反应最弱；ＰＡＳ反应患者组最强，成人、婴幼儿、胎儿依次减弱。结论：碱性磷酸酶随年龄活性逐渐降低，酸性磷酸酶次之，ＰＡＳ阳性物质随增龄而增多，说明椎间盘组织增长出现退行性改变

Aging induced histochemical and pathological changes in human intervertebral disc

Objective:To study anging induced histochemical and pathological changes in human intervertebral disc.Method:Acid phosphatase,alkaline phosphatase and PAS reaction of the cervical intervertebral disc in fetuses,infants,adults and patients with cervical spondylosis were observed with histochemical methods.Result:Acid phosphatase showed positive reaction in the patient group,negative reaction in the groups of fetuses and infants;the activity of alkaline phosphatase was the lowest in the group of patients and the highest in the group of fetuses;PAS stain showed very strong reaction in the group of patients,and gradually decreased respectively in the group of adults,infants and fetuses.Conclusion:The activity of alkaline phosphatase decreased with the age growing,while the acid phosphatase increased,PAS positive substance in the reaction of polysaccharide increased with aging.This research explained degeneration of intervertebral disc with the age growth.