参苓白术散对脾虚湿困型溃疡性结肠炎大鼠结肠组织ERK、p38 MAPK蛋白表达的影响

目的：探讨脾虚湿困型溃疡性结肠炎（Ulcerative Colitis，UC）大鼠结肠组织中ERK、p38MAPK蛋白表达的变化及参苓白术散的干预作用。方法40只Wistar大鼠随机分为正常对照组、模型对照组、柳氮磺吡啶组、参苓白术散组，每组10只。采用环境与饮食干预结合TNBS/乙醇灌肠法复制脾虚湿困型UC大鼠模型，应用免疫组织化学法检测各组大鼠结肠组织中ERK、p38MAPK蛋白表达情况。结果模型对照组大鼠结肠组织ERK、p38MAPK表达明显增多，与正常对照组比较具有显著性差异（P〈0.05）。柳氮磺嘧啶组和参苓白术散组大鼠结肠组织ERK、p38MAPK的表达与模型对照组比较差异有统计学意义（P〈0.05）。参苓白术散组大鼠结肠组织ERK、p38MAPK的表达与柳氮磺嘧啶组比较差异亦有统计学意义（P〈0.05或P〈0.01）。结论脾虚湿困型UC大鼠结肠组织ERK、p38MAPK表达明显增强，参苓白术散治疗可显著降低其ERK、p38MAPK的表达水平。

Effects on Expression of ERK,p38 MAPK in Ulcerative Colitis Rats with Syndrome of Dampness Stagnancy due to Spleen Deficiency Treated with Shenlinbaizhu Powder

Objective To investigate the effects on expression of ERK and p38MAPK in UC rats with syndrome of dampness stagnancy due to spleen deficiency that treated with Shenlinbaizhu powder. Methods 40 rats were divided into normal control group, model control group,Shenlinbaizhu group and sulfasalazine group,10 in each group. The model rats were induced by environment and diet intervention combine with composite TNBS and ethanol. The expression changes of ERK and p38MAPK in rats were detected by immunohistochemistry. Results The expression of ERK and p38MAPK in the model group was more than in normal group （P〈0. 05）. The expression of ERK and p38MAPK in sulfasalazine group and Shenlinbaizhu group significantly decreased and shown significant difference compared with those of model group（P〈0. 05）. There was significant difference between sulfasalazine group and Shenlinbaizhu group on the expression of ERK and p38MAPK （P〈0. 05）. Conclusion The expression of ERK,p38MAPK in the UC rats with syndrome of dampness stagnancy due to spleen deficiency was increased,and Shenlinbaizhu powder could significantly improve the expression of ERK,p38MAPK in the colon tissue of UC rats.