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Effects of chlorpromazine on the enzymatic and toxic properties of the most basic phospholipase A2 fromVipera russelli snake venom |
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| Authors: | A S Babu T V Gowda |
| Institution: | (1) Department of Studies in Biochemistry, University of Mysore, Manasagangotri, 570 006 Mysore, India;(2) Present address: Food Research Institute, Department of Food Microbiology and Toxicology, 1925, Willow Drive, University of Wisconsin, 53706 Madison, WI, USA |
| Abstract: | The effect of chlorpromazine on various biological activities of phospholipase A2 VRV-PL-VIIIa fromVipera russelli snake venom was investigated. The drug inhibited the in vitro phospholipase A2 activity of the enzyme by 55%. The ID50 was found to be 1.15 µM. Increasing substrate concentration relieved the inhibition of phospholipase A2 activity by the drug indicating probable interaction with the substrate. The drug totally quenched the fluorescence intensity of the enzyme. Chlorpromazine increased the LD50 of the enzyme by 1.6 fold. The drug also inhibited hemolytic and anticoagulant potencies but failed to inhibit edema inducing activity and myotoxicity of the enzyme. |
| Keywords: | Snake venom Phospholipase A2 chlorpromazine Interaction Inhibition |
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