活性氧在P，P’-DDE诱导大鼠睾丸支持细胞凋亡中的作用

目的]研究活性氧在2,2-双-（对氯苯基）-1，1-二氯乙稀（P,P’-DDE）诱导大鼠睾丸支持细胞凋亡中的作用。方法]从大鼠睾丸组织中分离支持细胞进行离体原代培养3d，用0、10、30、50μmol／L的p,p＇-DDE及加有300μmol／L的抗氧化剂N-乙酰-L-半胱氨酸（NAC）的P,P’-DDE（50μmol／L）继续培养24h，应用流式细胞仪测定细胞内活性氧（ROS）水平、线粒体膜势能（Aym）、凋亡发生率。结果]50μmol／L的P,P’-DDE可以诱导支持细胞ROS显著升高，10、30、50μmol／LP，P’-DDE处理组的线粒体膜势能下降，30、50μmol／L的P,P’-DDE可以诱导支持细胞凋亡，与对照组相比，差异有统计学意义（P〈0．05）；NAC有效抑制了ROS升高，削弱线粒体膜势能下降，减少了凋亡发生率，与50μmol／L的P,P’-DDE处理组相比，差异有显著意义（P〈0．05）。结论]P,P’-DDE可以诱导大鼠睾丸支持细胞凋亡，ROS产生可能是其重要原因之一。

The Role of Reactive Oxygen Species in P,P'-DDE-induced Apoptosis of Sertoli Cells in Rats

Objective ] To investigate the mechanism of p,p＇-DDE-induced apoptosis of Sertoli cells and the role of reactive oxygen species. Methods ] Sertoli cells from testes of rats were treated with 0, 10, 30, and 50 μmol/L p,p＇-DDE, or with 50 μmol/L p,p＇-DDE pretreated by 300 μmolFL NAC （ N-acetyl-L-cysteine ）. Then the fluoresence intensity of reactive oxygen species（ ROS ）, mitochondria membrane potential（ △ψm ）and apoptosis percentage was detected by a FACS Calibur flow cytometer. Results ] 50 μmol/L p,p＇-DDE increased the ROS level. The mitochondria membrane potential was decreased after all p,p＇- DDE＇s treatments. The percentage of apoptosis was increased after treatment with 30 or 50 μmol/L p,p＇-DDE. Compared with the control group, the difference was statistically significant（ P 〈 0.05 ）. The antioxidant NAC inhibited the increased ROS generation, decreased the mitochondria membrane potential and apoptosis percentage （ P 〈 0.05 ）. Conclusion ] p,p＇-DDE could induce apoptosis in Sertoli cells. The generation of ROS may be an important reason in the apoptosis.