| 复方肠泰抑制人结肠癌SW480细胞增殖的实验研究 |
| |
| 引用本文： | 张舒,沈明勤,丁永芳,王小宁.复方肠泰抑制人结肠癌SW480细胞增殖的实验研究[J].中成药,2010,32(3):374-378. |
| |
| 作者姓名： | 张舒 沈明勤 丁永芳 王小宁 |
| |
| 作者单位： | 江苏大学药学院;江苏省中医药研究院药理研究室; |
| |
| 基金项目： | 江苏省自然科学基金(BK2006156) |
| |
| 摘 要： | 目的:考察复方肠泰(人参、薏苡仁、莪术等)对人结肠癌细胞SW480细胞生长的抑制作用及其对细胞增殖周期和凋亡的影响。方法:采用噻唑蓝比色法测定不同浓度复方肠泰在不同作用时间内对在体外培养SW480细胞增殖的影响,同时应用流式细胞术检测细胞增殖周期及凋亡率的变化。结果:复方肠泰可在G0/G1期以及G2/M期阻滞人结肠癌细胞SW480的增殖,使S期细胞比率降低。结论:复方肠泰在3.51～7.81mg/mL范围内呈现明显的量效关系。
|
| 关 键 词： | 复方肠泰 人结肠癌细胞SW480 细胞周期 细胞增殖 凋亡 |
Antiproliferation of human colon carcinoma cell line (SW480) by Compound Changtai Granule |
| |
| ZHANG Shu,SHEN Ming-qin,DING Yong-fang,WANG Xiao-ning.Antiproliferation of human colon carcinoma cell line (SW480) by Compound Changtai Granule[J].Chinese Traditional Patent Medicine,2010,32(3):374-378. |
| |
| Authors: | ZHANG Shu SHEN Ming-qin DING Yong-fang WANG Xiao-ning |
| |
| Institution: | ZHANG Shu1,SHEN Ming-qin2,DING Yong-fang2,WANG Xiao-ning2(1.College of Pharmacy,Jiangsu University,Zhenjiang 212013 JIangsu,China,2.Dept.of Pharmacology,Jiangsu Institute of Traditional Chinese Medicine,Nanjing 210028,Jiangsu China) |
| |
| Abstract: | AIM:To check the antiproliferative and apoptosis-inducing effect of Compound Changtai Granule(Radix et Rhizoma ginseng,Semen coicis,Rhizoma curcumae,etc)(CCG) on human colon carcinoma cell line(SW480).METHODS:Methy thiazolyl tetrazolium chromatomerry was used to observe the influence of several concentration of CCG on SW480 cell proliferation cultured in vitro at the various times,and flow cytometry was applied to examining the change in SW480 cell proliferation cycle and apoptosis rate.RESULTS:CCG could su... |
| |
| Keywords: | Compound Changtai Granule human colon carcinoma cell line SW480 cell Cycle cell proliferation apoptosis |
| 本文献已被 CNKI 等数据库收录！ |
|
