Modification of the mouse mammary gland organ culture technique |
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| Authors: | R. G. Mehta W. L. Cerny M. J. Madigan R. C. Moon |
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| Affiliation: | (1) Laboratory of Pathophysiology, Life Sciences Research Division IIT Research Institute, 60616 Chicago, Illinois, USA |
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| Abstract: | Summary A modified procedure for culturing an entire thoracic pair of mammary glands from virgin mice is described. The glands are incubated in CMRL 1066 medium supplemented with hormones in a controlled atmosphere chamber with a gas phase of 50% O2, 5% CO2, and 45% N2 at 37° C. |
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| Keywords: | mammary gland differentiation hormones |
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