高迁移率族蛋白B1作为电离辐射生物剂量计的初探

目的 初步研究高迁移率族蛋白B1 (high mobility group box-1,HMGB1)作为电离辐射生物剂量计的可能性。 方法 体外培养的正常人成纤维母细胞系GM接受不同剂量⁶⁰Co γ线照射后,于照后24 h收集培养液上清,经ELISA法检测其中HMGB1蛋白含量的变化,并建立剂量-效应曲线。同时采用4 Gy γ线照射后于24、48和72 h检测培养液中HMGB1的含量变化。结果 细胞培养液上清中HMGB1蛋白的含量随辐射剂量的增加而增加,照后24 h的剂量-效应曲线符合线性模式y= 0.5655 +0.0358x(r=0.9339)。且培养液上清中HMGB1蛋白含量随照后培养时间的延长而增加。结论 HMGB1是电离辐射反应性蛋白,对其进行深入研究将有助于HMGB1作为辐射生物剂量计早日应用于辐射损伤的防护与救治。

Preliminary study on high mobility group box 1 as a biological dosimeter of ionizing radiation

Objective To investigate the probability of high mobility group box 1 protein as a biological dosimeter of ionizing radiation. Methods The medium of cultured human fibroblast cell line GM was collected 24 h after exposed to 60Co γ-rays, and HMGB1 protein concentration was detected by ELISA assay, and the dose-effect curve was then fitted. The levels of HMGB1 protein was also detected after exposed to 4 Gy irradiation at 24,48 and 72 h. Results The level of HMGB1 protein in culture medium was increased in a dose-dependant way, and the dose-effect curve of HMGB1 level for 24 h after irradiation fitted the linear model y = 0.5655 + 0.0358x (r = 0. 9339) . After exposure to 4 Gy irradiation, the levels of HMGB1 protein in the culture medium were also increased time-dependently.Conclusion The release of HMGB1 protein to neoplasm is reactivated by irradiation in GM cell. It is necessary to further the studies on HMGB1 as biological dosimeter for radiation injury protection and therapy.