体外诱导大鼠骨髓间充质干细胞分化为心肌样细胞

目的] 探讨体外培养定向诱导大鼠骨髓间充质干细胞 ( MSCs )向心肌样细胞分化的潜能.方法] 取大鼠下肢骨骨髓,分离培养 MSCs,用 5-氮胞苷 ( 5-aza )定向诱导 24 h,相差显微镜下观察其形态变化,免疫组化法检测肌钙蛋白 ( cTnT )和心肌细胞结蛋白 ( desmin )表达,并通过 RT-PCR对肌球蛋白重链 ( MHC )在细胞中的表达进行鉴定.结果] MSCs经 5-aza诱导后,部分细胞呈梭形,并可见肌管样结构.免疫组化检测示诱导后 MSCs的 cTnT阳性细胞数为 15%, Desmin表达强阳性, RT-PCR示诱导后 MSCs有 MHC表达.结论] MSCs在体外能在一定条件下被诱导分化为心肌样细胞,是心肌缺血干细胞移植的较理想的细胞来源.

Study on Induction of Rat Mesenchymal Stem Cells to differentiate into Cardiomyocytes in vitro

Objective] To study the potential of rat mesenchymal stem cells ( MSCs ) to differentiate into cardiomyocytes by induction in vitro. Methods] SD rat MSCs were isolated and cultured from rat bone marrow, then induced by 5-aza for 24 h. The MSCs differentiation was observed by phase-contrast microscope. The immunohistochemical technique was used for the expression of cTnT and desmin. The expression of myocin heavy chain ( MHC ) was examined by RT-PCR. Results] After being induced by 5-aza, some of MSCs became spindle-like and formed myotube-like structure. And cTnT positive cells were about 15%, desmin positive cells were found by immunohistochemistry staining. The expression of MHC ( one of cardie-special genes ) could be detected by RT-PCR. Conclusion] The MSCs can be differentiated to cardiomyocytes in vitro by 5-aza, and MSCs might be a new cell source of stem cell transplantation in ischemic myocardium.