| 矿化液对人脂肪基质细胞(hADSCs)成骨分化的影响 |
| |
| 引用本文： | 张勇,刘洪臣,鄂玲玲,王东胜.矿化液对人脂肪基质细胞(hADSCs)成骨分化的影响[J].口腔颌面修复学杂志,2010,11(2). |
| |
| 作者姓名： | 张勇 刘洪臣 鄂玲玲 王东胜 |
| |
| 作者单位： | 解放军总医院口腔医学研究所,北京,100853 |
| |
| 摘 要： | 目的:比较人脂肪基质细胞(human adipose-derived mesenehymal stem cells,hADSCs)在矿化条件与非矿化条件下分别培养,评价矿化液对其增殖和分化的影响。方法:酶消法原代培养人脂肪基质细胞,再以含地塞米松、β-甘油磷酸钠和维生素C的矿化液进行诱导,MTT检测矿化液对细胞增殖的影响,细胞培养上清液检测碱性磷酸酶(ALP)活性及骨钙素(OCN)含量。结果:矿化液抑制细胞的增殖,提高人脂肪基质细胞ALP活性和骨钙素的含量。结论:人脂肪基质细胞在矿化条件下可以表现出成骨细胞特性。
|
| 关 键 词： | 人脂肪基质细胞 矿化液 分化 |
Osteogenic differentiation of human adipose-derived stem cells by mineralization solution |
| |
| ZHANG Yong,LIU Hong-chen,E Ling-ling,WANG Dong-sheng.Osteogenic differentiation of human adipose-derived stem cells by mineralization solution[J].Chinese Journal of Prosthodontics,2010,11(2). |
| |
| Authors: | ZHANG Yong LIU Hong-chen E Ling-ling WANG Dong-sheng |
| |
| Institution: | ZHANG Yong; LIU Hong-chen; E Ling-ling; WANG Dong-sheng. (Stomotological institute of Chinese PLA general hospital; Beijing 100853; China); |
| |
| Abstract: | Objective:Toexpl oreosteogenic differentiation of human adipose-derived mesenehymal stem cells bymineralization solution. Methods: Human liposuction aspirates were proteolytically digested and centrifuged to obtain hADSCs. After primary culture in control media and expansion to four passages, the cells were cultured in osteogenic media and control media for 4 weeks respectively. Then the cultured cells was assessed by MTT, OCN content and alkaline phosphatase activity. Results: The cells’proliferation was i... |
| |
| Keywords: | hADSCs mineralization solution differentiation |
| 本文献已被 维普 万方数据 等数据库收录！ |
|
