| 人骨髓间充质干细胞体外培养及与Bio-gide胶原膜复合培养的实验研究 |
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| 引用本文： | 宋莉,曲丰江,汪泱,王冰,戴芳.人骨髓间充质干细胞体外培养及与Bio-gide胶原膜复合培养的实验研究[J].江西医学院学报,2009,49(5). |
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| 作者姓名： | 宋莉 曲丰江 汪泱 王冰 戴芳 |
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| 作者单位： | 南昌大学第二附属医院口腔科 |
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| 基金项目： | 2005年江西省科技厅科学技术研究项目 |
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| 摘 要： | 目的本实验拟研究人骨髓间充质干细胞(human mesenchymal stemcells,hMSCs)分离、培养与鉴定的方法,探讨其作为牙周组织工程种子细胞的可行性。方法体外分离培养hMSCs,进行形态学观察,细胞表面抗原检测;将hMSCs与Bio-gide胶原膜进行体外复合培养,通过倒置相差显微镜,扫描电镜进行观察。结果梯度密度离心法结合贴壁筛选法能获得高纯度高活性的hMSCs。hMSCs可在Bio-gide胶原膜上贴附增殖,并复层生长。结论hMSCs取材方便、创伤小;扩增分化能力强。hMSCs可作为牙周组织工程种子细胞来源。Bio-gide胶原膜可作为细胞生长载体。
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| 关 键 词： | 骨髓间充质干细胞 Bio-gide胶原膜 组织工程 |
Culture of Human Bone Marrow-derived Mesenchymal Stem Cells and Its Culture on Bio-gide Collagen Membrane in Vitro |
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| SONG Li; QU Feng-jiang; WANG Yang; WANG Bin; DAI Fang.Culture of Human Bone Marrow-derived Mesenchymal Stem Cells and Its Culture on Bio-gide Collagen Membrane in Vitro[J].Acta Academiae Medicinae Jiangxi,2009,49(5). |
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| Authors: | SONG Li; QU Feng-jiang; WANG Yang; WANG Bin; DAI Fang |
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| Institution: | SONG Li; QU Feng-jiang; WANG Yang; WANG Bin; DAI Fang(Department of Stomatology; the Second Affiliated Hospital ofNanchang University; Nanchang 330006; China); |
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| Abstract: | Objective The purpose of this paper is to assess the feasibility of making human mesenchymal stem cells(hMSCs) the seed cells of periodontal tissue engineering through investigation into the methods of separation,culture and identification of(hMSCs),as well as its culture on Bio-gide collagen membrane in vitro.Methods HMSCs were separated and cultured in vitro,whose morphological characteristics were observed and the cell surface antigen was detected.HMSCs were also cultured on Bio-gide collagen membrane in... |
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| Keywords: | mesenchymal stem cell bio-gide collagen membrane tissue engineering |
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