血清葡萄糖-6-磷酸异构酶在类风湿性关节炎中的应用

目的：探讨血清中葡萄糖-6磷酸异构酶（G6PI）对类风湿性关节炎（RA）诊断及与其他自身免疫性疾病鉴别诊断中的价值。方法：采用酶联免疫吸附试验（ELISA）检测137例RA患者、141例其他风湿病患者和60例健康对照血清中GPI抗原的浓度，RA患者还检测了抗环胍氨酸肽抗体（抗CCP抗体）及类风湿因子（RF）的浓度。结果：137例RA患者血清中GPI浓度为（3．01±1．33）mg／L，141例其他风湿性疾病组为（0．27±0．16）mg／L，60例健康对照组为（0．07±0．03）mg／L。RA患者血清中GPI浓度显著高于其他风湿病组和健康对照组（P〈0．01）；在RA活动组和RA非活动组中亦差异有统计学意义（P〈0．01）。GPI抗原对RA诊断的敏感性为62．8％，特异性为95．7％。3个指标联合检测的特异性达100％。结论：GPI在部分RA患者血清中显著升高，有可能成为诊断RA及判断其疾病活动性的一个新指标。

Applicaton of serum glucose-6-phosphate isomerase in diagnosis of rheumatoid arthritis

Objective：To explore the diagnostic value of glucose-6-phosphate isomerase（GPI） in rheumatoid arthritis（RA） and other autoimmune diseases. Method： The level of serum GPI in 137 patients with RA, 141 patiens with other rheumatisms and 60 normal controls were detected by ELISA. The level of anti-cyclic citrullinated peptide antibody and rheumatoid factor （RF） were also detected in RA patients. Result：The average GPI serum levels in 137 patients with RA, 141 patients with other rheumatisms and 60 mormal controls were（3.01±1.33） mg/L, （0.27±0.16） mg/L and （0. 07±0.03） mg/L, respectively. The GPI serum level in RA patients was significantly higher that that of patients with other rheumatisms and 60 mormal controls （ P 〈0.01）. There were significant statistical differences between active RA and inactive RA （ P 〈0.01）. The sensitivity and the specificity of GPI for RA were 62. 8% and 95. 7%, respectively. The specificity of combind tests of these three indexes reached 100%. Conclusion：GPI might be a new marker for diagnosing RA and accessing its activity which significantly in- creased in some RA patients.