骨髓间质干细胞移植对梗死心肌的影响

目的探讨自体骨髓间质干细胞移植对梗死心肌基本结构及心肌收缩力的影响。方法贵州香猪24只,随机分为对照组和实验组,每组12只。抽取骨髓3 mL,按照W ak itan i方法培养出骨髓间质干细胞,经5-氮胞苷诱导后,5-溴脱氧尿苷标记备用。开胸结扎左冠状动脉前降支后,实验组第三代自体骨髓间质干细胞分别注射至结扎的左冠状动脉前降支和远端多点注入急性心肌梗死区域;对照组以同样方法注射DMEM。术后3、6周取标本,组织切片染色,观察组织基本结构;体外药物刺激离体肌条,检测心肌收缩情况。结果VG染色显示实验组胶原纤维融合较少,组织结构排列基本处于有序状态。实验组心肌收缩力明显高于对照组(P<0.001),且随时间推移恢复程度增加(P<0.05)。结论自体骨髓间质干细胞梗死心肌移植可以增加心肌细胞的收缩力,调节组织内胶原纤维的含量和组成,防止心室重构,从而起到对心肌基本结构的保护作用。

Effect of Implantation of Bone Marrow Mesenchymal Stem Cells on Infarcted Myocardium

Objective To study the effect of implantation of bone marrow mesenchymal stem cells(MSCs)on infarcted myocardium. Methods Twenty-four Guizhou Xiang porcine were equally divided into an experimental group and a control group randomly.3 mL bone marrow was extracted from the posterior superior iliac spine.MSCs were cultured according to the methods of Wakitani's.After being co-cultured with 5-azacytidine for 24 h,these cells were labeled with bromodeoxyuridine.Autologus MSCs were implanted into the acute myocardial infarct site both via the distal segment of the ligated left anterior descending artery and topical injection.Specimens and muscle strip were harvested at 3 and 6 weeks after MSCs implantation for morphologic examinations and contractile force study. Results The structure in the experimental group appeared in an orderly arrangement as compared with that in the control group.The collagenous fibers fused less and the basic structures appeared in an orderly arrangement in the experimental group as showed by VG staining.The contractile force in the experimental group was better than that in the control group(P<0.001). Conclusion After being implanted into infarct myocardium,MSCs showed the ability of structural preservation of myocardial tissue and improvement of the contractile perfor-(mance) in the infarcted myocardium.