DNA damage and repair in human leukocytes exposed to styrene-7,8-oxide measured by the comet assay. |
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Authors: | Blanca Laffon Eduardo Pásaro Josefina Méndez |
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Affiliation: | 1. Division of Pulmonology and Allergy, Department of Internal Medicine, Gachon University Gil Medical Center, Incheon, Republic of Korea;2. Department of Biomedical Chemistry, KonKuk University, Chungju 27478, Republic of Korea;3. Gachon Medical Research Institute, Gachon University Gil Medical Center, Incheon, Republic of Korea;4. Division of Pulmonary and Critical Care Medicine, Joan and Sanford I. Weill Department of Medicine, Weill Cornell Medicine, New York, NY, USA;5. Department of Biochemistry, College of Medicine, Gachon University, Incheon, Republic of Korea |
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Abstract: | Styrene-7,8-oxide (SO) is produced by cytochrome p450 monooxygenases as the main mammalian metabolite of styrene, an important industrial chemical present at high concentrations in the ambient air of fiberglass-reinforced plastic plants. Previous studies have shown positive results for SO in the induction of several cytogenetic endpoints in vitro. In this work we have evaluated, by means of the comet assay, the potential of SO to act as a DNA damaging agent in human peripheral leukocytes and the ability of white blood cells to repair the DNA damage induced by this compound. Our results show that SO induces DNA damage at concentrations higher than 50 microM in a dose-dependent manner, and that the lesions produced by SO are efficiently removed within a few hours after the end of treatment. |
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