泪腺多形性腺瘤或癌DNA含量和P^53基因产物表达的定量分析

应用流式细胞计量术和免疫荧光染色技术,对１８例多形性腺瘤和６例多形性腺癌细胞的ＤＮＡ含量及Ｐ５３基因产物表达进行定量测定,探讨其在该肿瘤病理学诊断中的意义。结果表明,多形性腺瘤具有正常泪腺细胞的ＤＮＡ二倍体含量;多形性腺癌以ＤＮＡ异倍体为特征;Ｐ５３...

Quantitative analysis of DNA content and P 53 gene product expression from pleomorphic adenoma and carcinoma arising in pleomorphic adenoma

Flow cytometry(FCM) and immunofluorescence staining technique were used to quantitatively measure the DNA content and P 53 gene product expression from 18 cases of pleomorphic adenoma and 6 cases of carcinoma arising from pleomorphic adenomas,in order to detect the significance of quantitative analysis of two parameters applied for pathological diagnosis of pleomorphic adenoma and carcinoma arising from pleomorphic adenoma.The results showed that the pleomorphic adenoma possessed the properties of DNA diploid of normal lacrimal gland cells,but the carcinoma arising from pleomorphic adenomas were characterized by DNA aneuploidy.The significant difference of P 53 gene product expression between the two kinds of tumors was observed.DNA content was positively correlated to the quantity of P 53 gene product expression.It was demonstrated that the quantitative determination of DNA content and P 53 gene product expression may provide objective indexes for the pathological diagnosis of this tumors.