| Ad-huVEGF121基因转染对兔坏死股骨头血流的影响 |
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| 引用本文： | 贾勇,初同伟,周跃,罗刚.Ad-huVEGF121基因转染对兔坏死股骨头血流的影响[J].第三军医大学学报,2005,27(9):885-887. |
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| 作者姓名： | 贾勇 初同伟 周跃 罗刚 |
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| 作者单位： | 第三军医大学新桥医院骨科,重庆,400037;第三军医大学新桥医院骨科,重庆,400037;第三军医大学新桥医院骨科,重庆,400037;第三军医大学新桥医院骨科,重庆,400037 |
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| 摘 要： | 目的探讨一种治疗股骨头缺血坏死的新方法.方法重组腺病毒Ad-hVEGF121注射入坏死的股骨头,应用RT-PCR和免疫组织化学技术检测VEGF121的表达,观察股骨头内新生血管数量,用SPECY技术观察坏死股骨头的动脉灌注情况.结果转染后的股骨头内VEGF121表达增高,新生血管数量增多,SPECT观察发现坏死股骨头血流量明显增加.结论转染VEGF1:1基因可以增加坏死股骨头新生血管数量和血流量.
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| 关 键 词： | 血管内皮细胞生长因子 股骨头坏死 |
| 文章编号： | 1000-5404(2005)09-0885-03 |
| 修稿时间： | 2005年1月18日 |
Enhancing blood flow of necrotic femoral head by adenovirus hu-VEGF121 transfection in rabbits |
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| Jia Yong,CHU Tong-wei,ZHOU Yue,LUO Gang.Enhancing blood flow of necrotic femoral head by adenovirus hu-VEGF121 transfection in rabbits[J].Acta Academiae Medicinae Militaris Tertiae,2005,27(9):885-887. |
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| Authors: | Jia Yong CHU Tong-wei ZHOU Yue LUO Gang |
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| Abstract: | Objective To explore a new method for the therapy of vascular necrosis of the femoral head. Methods The recombinant Ad-huVEGF 121 was implanted into the necrotic femoral head. The expression of vascular endothelial growth factor (huVEGF 121 ) was examined by RT-PCR and immunohistochemical techniques. Angiogenesis was observed by histomorphometric analysis and blood flow of femoral head was observed by SPECT. Results The expression of huVEGF 121 was detected in the femoral head transfected with the huVEGF 121 gene. The femoral head transfected with the huVEGF 121 gene showed a significant increase in angiogenesis and blood flow of femoral head after gene transfection. Conclusion Transfection of the VEGF 121 gene enhances angiogenesis of bone tissue and blood flow of necrotic femoral head. |
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| Keywords: | vascular endothelial growth factor femoral head necrosis |
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