妊娠高血压疾病患者血清及胎儿脐血VE-cadherin和HMGB1检测的临床意义

目的 研究妊娠期高血压疾病患者静脉血清及胎儿脐血中血管内皮细胞钙黏蛋白（VE-cadherin） 和高迁移率蛋白-1（HMGB1）的变化及意义.方法 采用酶联免疫吸附法（ELISA）进行VE-cadherin和HMGB1含量的测定,分别测定90例妊娠期高血压疾病患者（其中妊娠期高血压、轻度子痫前期、重度子痫前期各30例）及30例正常对照组产妇静脉血清及胎儿脐血中VE-cadherin和HMGB1的含量.结果 VE-cadherin和HMGB1在妊娠期高血压疾病组及对照组血清及胎儿脐血中均有表达,VE-cadherin在妊娠期高血压疾病各组血清中含量与对照组血清中的比较,差异有显著意义（t=7.31,9.82,10.04,P值均〈0.05）,且重度子痫前期组明显高于轻度子痫前期和妊娠期高血压组（t=7.23,8.95,P值均〈0.05）;轻度子痫前期组明显高于妊娠期高血压组（t=7.68,P〈0.05）.HMGB1在妊娠期高血压疾病各组血清中含量与对照组血清中的比较,差异有显著意义（t=7.54,9.65,10.18,P值均〈0.05）,且重度子痫前期组明显高于轻度子痫前期和妊娠期高血压组（t=7.54,9.65,P值均〈0.05）;轻度子痫前期组明显高于妊娠期高血压组（t=7.35,P〈0.05）.妊娠期高血压疾病患者VE-cadherin HMGB1含量呈正相关（r=0.59,P〈0.01）.VE-cadherin和HMGB1在妊娠期高血压疾病各组胎儿脐血中的含量,差异无显著意义（P〉0.05）.结论 VE-cadherin和HMGB1的高表达说明,妊娠期高血压疾病患者内皮细胞损伤,且VE-cadherin和HMGB1在损伤过程中起作用.

The clinical significance of VE-cadherin and HMGB1 detection in serum and fetus umbilical cord blood of patients with hypertensive disorders in pregnancy

Objective To investigate the expression and significance of VE - eadherin and high mobility group box - 1 （HMGB1） in intravenous serum and umbilical cord blood of patients with hyperlensive disorders in pregnancy （pregnancy- induced hypertension syndrome, PIH）. Methods Contents of VE - cadherin and HMGBI in intravenous serum and umbilical cord blood from 90 patients with hypertensive disorders in pregnancy （which were divided into gestational hypertension, mild and severe preeclampsia groups each including 30 patients） were detected by enzyme- linked immunosorbent assay（ELISA） respectively. 30 normal pregnant women were taken as control group. Results VE - cadherin and HMGB1 expressed in both patients and controls. Serum contents of VE - cadherin in padents and controls had significant differenee by comparison （t = 7.31,9.82,10.04,all P 〈 0.05）. Moreover, there were differences among the contents of three groups（ t = 7.23, 8.95,7.68, all P 〈 0.05）. Sernm contents of HMGB1 in patients and controls had significanl difference by comparison （t = 7.54,9.65, 10. 18, all P 〈 0.05）. Moreover, there were differences among the contents of three groups （ t = 7.54,9.65,7.35, all P 〈 0.05 ）. Conclusion of VE - cadllerin and HMGB1 of patients with hypertensive disorders in pregnancy showed a positive correlation （r = 0.59, P 〈 0.01 ）. There were no significant differences in contents of VE - cadherin and HMGB1 in umbilical cord blood of patients with hypertensive disorders in pregnancy in three groups（ P 〉 0.05 ）. Conclusion The high expression of VE - cadherin and HMGBI in patients with hypertensive disorders in pregnancy indicales the injury_ of vascular endothelium cells and VE - eadherin and HMGB1 play a role in the process of iniury.