同种异体黑素细胞移植治疗白癜风

目的：探索同种异体纯培养黑素细胞移植治疗白癜风的可行性。方法：用负压吸疱法在一位女性稳定期白癜风志愿白斑妪吸疱，注入来自健康男性供体的培养黑素细胞悬液，连续观察外观效果，并通过组织病理学和Y染色体特异性片段检测的方法确定异体黑素细胞的转归。如果移植区色素有一定恢复，则重复实验，检测结果的可靠性。结果：首例患移植一月后移植区有色素开始恢复，75天后颜色与邻近正常皮肤颜色接近。160天后复色区组织病理显示基底层有黑素细胞存活，并向周围的角质形成细胞分泌大量黑素颗粒；组织DNA中检查出有Y染色体特异性片段。再次移植另一个供体的黑素细胞，移植区域也有明显色素恢复。而原先恢复的色素仍然稳定存在。在其他志愿中有的也重复出了类似结果。结论：同种异体黑素细胞移植可以在白癜风患身上存活，使皮肤恢复颜色。它有希望成为治疗白癜风的一个新方法。

Explore of allogenic melanocytes transplantation on vitiligo

AIM To study the feasibility of vitiligo treatment through allogenic transplantation of pure melanocytes. METHODS By negative pressure suction blisters were made at a female vitiligo volunteer's lesions. Suspension of pure cultured melanocytes from a healthy male donor was injected into the blisters. The effects in appearance were observed continuously. The outcome of the allogenic melanocytes was determined by pathological examination and specific fragment of Y chromosome detection. After color recovery were observed at the transplanted areas, ten volunteers joined the test too. RESULTS The four transplanted spots' colors of the first female transplanter appeared some re pigmentation 1 mo after transplantation. The transplant spots' colors were almost identical to those of adjacent normal skin 2 mo later. One hundred and sixty days later pathological examination of one repigmented spot displayed that there were melanocytes that could secrete melanin into adjacent keratinocytes. Y chromosome fragment was detected positive in the DNA extracted from the skin of another repigmented spot. Two hundred days after second grafting one transplanted spot showed obvious color recovery too. Similar results were observed in some of other transplanted patients. CONCLUSION Because the allogenic melanocytes can survive in acceptor grafting of cultured allogenic melanocytes and can repiment vitiligo lesions and it is promising to develop a new way to treat vitiligo.