颌面爆炸伤早期愈合过程中血管内皮生长因子及其受体表达的实验研究

目的 分析颌面部软组织爆炸伤早期愈合过程中血管内皮生长因子（VEGF）胶受体F1t－1的表达和变化，探讨VEGF在上述损伤愈合过程中的作用。方法 采用改进的家兔颌面部爆炸伤动物模型，以KTY－04型雷管为爆炸源，复制颌面部爆炸损伤。选用ABC免疫组化染色法分别检测伤后6h、1d、2d、3d、5d和7d伤口组织中VEGF及其受体Flt－1的表达，并与伤前组比较。结果 颌面部爆炸伤伤口组织中VEGF表达在伤后第一周内稳步上升，于伤后第1d起和伤前组比较，差异明显，伤后第3d开始，差异非常显著（P＜0．01），伤后7d达峰值。VEGF受体Flt－1的表达则在伤后3d内与伤前组比较无显著差异，伤后5－7d其蛋白质的表达明显加强。结论 VEGF在颌面部爆炸伤后的表达与创伤愈合过程中新生血管形成的时间相似，其受体Flt－1的表达也多发生于此时期，说明VEGF参与调节了创伤愈合过程中的血管再生阶段，对毛细血管的发生有积极促进作用。

Expression of vascular endothelial growth factor during the early stage of maxillofacial blast injury

Objective To determine the expression and the change of vascular endothelial growth factor(VEGF) and it's acceptor Flt-1 during maxillofacial blast injury at early stage,and to evaluate the effect of VEGF on traumatic wound healing.Methods The rabbit model of maxillofacial blast injury was made by KTY-04 blasting cap. The expression of VEGF and Flt 1 in wound tissue was determined by ABC immunohistochemistry after injury 6h,1d,2d,3d,5d,and 7d. Also,the results were compared with preinjury groups.Results In the wound tissue of maxillofacial blast injury, the expression of VEGF was rising steadily at the first week after injury. Comparing with normal tissue,it showed different from the first day after injury, and showed very different from the third day(P<0.01).It reached the peak at the seventh day after injury.The expression of Flt 1 showed no difference within 3 days after injury comparing with normal tissue.After injury 5d-7d,the protein expressed strengthly.Conclusion The stage of the VEGF expression at maxillofacial blast injury is similar to the angiogenic stage during wound healing, and the Flt 1 expression is also occurring at that period.It illustrates that VEGF take part in angiogenic cascades of traumatic wound healing and product auxo action to the regeneration of blood vessel.