Proteoglycan biosynthesis by rabbit articular chondrocytes treated withd-penicillamine |
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Authors: | P Legendre M Bouakka M Langris J -P Pujol R Beliard G Loyau J Bocquet |
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Institution: | (1) Laboratoire de Biochimie du Tissu Conjonctif, U.F.R. de Sciences, C.H.U. Côte de Nacre, 14032 Caen cedex, France;(2) Laboratoire d'Anatomie Pathologique, U.F.R. de Médecine, C.H.U. Côte de Nacre, 14032 Caen cedex, France |
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Abstract: | Rabbit articular chondrocytes in confluent monolayer cultures were treated withd-Penicillamine (d-Pen) during 3 or 5 days. The 35S]-sulfate incorporation in neosynthesized proteoglycans was not modified byd-Pen doses ranging from 50 to 800 g/ml. After treatment during 5 days withd-Pen concentrations of 50 or 400 g/ml, the chemical characteristics of proteoglycans from medium and cell-layer were determined. The aggregation capacity of proteoglycans from medium, the monomer molecular size, the glycosaminoglycan chain length and the relative rates of the different glycosaminoglycans (chondroitins, chondroitin 6-sulfate, chondroitin 4-sulfate, hyaluronic acid) remained unchanged. These results suggest thatd-Pen does not alter some of the cartilage mechanical properties due to the presence of proteoglycans. |
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