载银磷酸锆纱布对烧伤创面常见细菌作用的实验研究

目的 了解载银磷酸锆纱布对烧伤大鼠创面感染常见细菌的抗菌效力。方法制作Wistar大鼠烫伤(以下称烧伤)感染模型，采用试管双倍稀释法测定载银磷酸锆及磺胺嘧啶银的最低抑菌浓度(MIC)及最低杀菌浓度(MBC)。观察大鼠创面用磺胺嘧啶银纱布、普通尢菌纱布、载银磷酸锆纱布(小水洗及水洗20次)后痂下组织的细菌计数和对创面愈合的作用。结果载银磷酸锆对金黄色葡萄球菌、铜绿假单胞菌、大肠竹菌的MIC各为8、8、16mg／L，MBC各为16、8、32mg／L，为磺胺嘧啶银的2～8倍?大鼠创面用未水洗的载银磷酸锆纱布覆盖后，痂下细菌计数比用磺胺嘧啶银纱布低91％，比普通无菌纱布低99％未水洗的载银磷酸锆纱布对创面愈合的作用明显优于其他两种纱布，且其痂下组织细菌计数与水洗20次的效银磷酸锆纱布比较，差异无显著性意义(P＞0．05)。结论载银磷酸锆埘烧伤创面感染常见细菌有较强的抑制和杀灭作用，其敷料可作为治疗措施中新的选择。

Experimental study on the antibacterial effects of zirconium phosphate gauze loaded with silver on the common bacteria on rat burn wound

Objective To investigate the antibacterial effects of zirconium phosphate gauze loaded with silver on rat burn wounds seeded with commonly seen bacteria. Methods Wistar rats were employed in the study and were scalded and infected.The minimum inhibition concentration (MIC) and minimum bactericidal concentration (MBC) of zirconium phosphate loaded with silver were determined by double dilution in tubes.The effect on wound healing and the subeschar bacterial count of the rat burn wounds were observed after the wounds had been covered by gauze loaded with zirconium phosphate and silver ,and also with the gauze which has been rinsed for 20 times. Results The MIC of silver loaded zirconium phosphate on Staphylococcus aureus, Pseudomonas aeruginosa and E.coli were 8,8 and 16 mg/L, respectively, while the MBC were 16, 8 and 32 mg/L, respectively.The subeschar bacterial count in the burn wounds with the gauze with silver loaded zirconium phosphate was ten times lower than that in those which were treated with gauze with SD-Ag and 100 times lower than that with ordinary gauze. But there was no difference in the bacterial count between the wounds which were treated with fresh gauze with silver loaded zirconium phosphate and that with the gauze which has been rinsed for 20 times (P>0.05). Furthermore, wound healing seemed to be better with the gauze with silver loaded zirconium phosphate when compared with those by the other two kinds of gauze. Conclusion The silver loaded zirconium phosphate was found to be bacteriocidal against bacteria commonly seen in the burn wounds.