PCNA及Ki67在脑星形细胞瘤中的表达

为探讨增生细胞核抗原 (PCNA)及Ki67在脑星形胶质细胞瘤表达的临床意义 ,应用SP免疫组织化学染色法检测 3 8例不同级别的星形细胞瘤标本PCNA及Ki67的表达。发现PCNA标记指数 (LI)除在星形细胞瘤Ⅲ级和Ⅳ级组间无显著性差异外 ,其他各级别组间均存在显著性差异 (P <0 .0 5 ) ,随肿瘤恶性程度的增加而增高 ;Ki67的表达总体差异具有显著性 (P <0 .0 5 ) ,但在Ⅰ与Ⅱ级别组间、Ⅲ与Ⅳ级别组间均无显著性差异 ;PCNALI与肿瘤体积成正比 (r =0 .97,P <0 .0 5 )。提示蛋白水平半定量检测脑星形细胞瘤PCNA的表达情况 ,结合病理学分级 ,可以客观地反映肿瘤的增生潜能、生长方式和组织学性质等生物学特性。

Expression of PCNA and Ki67 in Brain Astrocytomas

To elucidate the clinical value of expression of proliferating cell nuclear antigen(PCNA)and Ki67 in brain astrocytomas, the immunostaining of PCNA and Ki67 in 38 astrocytomas were performed by Streptavidin-Peroxidase(SP)method. The result showed the difference of PCNA labeling index(PCNA LI)between tumor grades were statistically significant(P<0.05)except those in group Ⅲ and group Ⅳ. In general,PCNA LI increased with tumor grade. Though total variance of Ki67 LI among tumor grades was remarkable(P< 0.05), there were no significant difference among groupⅠ and Ⅱ and group Ⅲ and Ⅳ. The mean tumor volume had a positive correlation with PCNA LI(r=0.97, P< 0.05). It can be concluded that the expression of PCNA at protein level assessed half quantitatively by immunohistochemistry combined with pathology seem to give reliable information about biological behavior including proliferative potential, growth patterns and histological feather of astrocytomas.