Evaluation of a multiplex PCR system for simultaneous detection of Salmonella spp., Listeria monocytogenes, and Escherichia coli O157:H7 in foods and in food subjected to freezing |
| |
| Authors: | Kawasaki Susumu Fratamico Pina M Horikoshi Naoko Okada Yukio Takeshita Kazuko Sameshima Takashi Kawamoto Shinichi |
| |
| Institution: | Food Hygiene Laboratory, National Food Research Institute, Tsukuba, Japan. |
| |
| Abstract: | Conventional culture methods were compared to a multiplex polymerase chain reaction (PCR) assay for simultaneous detection of Salmonella spp., Listeria monocytogenes, and Escherichia coli O157:H7 from enrichment cultures of various types of artificially inoculated and naturally contaminated foods. The multiplex PCR assay was evaluated in 44 types of spiked food samples, including meat, produce, fish, and dairy products targeting genes specific for each pathogen for simultaneous detection. The sensitivity of the assay was
|
| |
| Keywords: | |
| 本文献已被 PubMed 等数据库收录！ |
|
