碱性成纤维细胞生长因子对皮肤萎缩性瘢痕中成纤维细胞的作用

目的探讨人碱性成纤维细胞生长因子(bFGF)对豚鼠皮肤萎缩性瘢痕中成纤维细胞的作用。方法取实验用成年豚鼠20只,于脊柱旁两侧A,B,C三处皮肤分别人工造成萎缩性瘢痕后,实验组在A处真皮层注射50μg/L的bFGF0.1mL,隔日1次,共4次;在C处真皮层注射同剂量生理盐水作阴性对照,B处不作任何处理做空白对照。术后第21天切取瘢痕组织行病理切片,应用鼠抗人ki-67单克隆抗体行免疫组化,显微镜下计算增殖成纤维细胞所占百分率。结果实验组、阴性对照组和空白对照组切口瘢痕增殖成纤维细胞百分率分别为(7.63±1.42)%,(0.98±0.33)%和(1.22±0.34)%。实验组增殖成纤维细胞百分率与阴性对照组及空白对照组比较显著增高,差异有统计学意义(P<0.05)。结论 bFGF可以促进豚鼠皮肤萎缩性瘢痕中的成纤维细胞增殖。

Effect of Basic Fibroblast Growth Factor on Fibroblasts in Cutaneous Atrophic Scars

Objective To investigate the functions of human basic fibroblast growth factor on fibroblasts of guinea pigs’ cutaneous atrophic scars. Methods Three atrophic scars marked A,B and C were chosen from the dorsal skin of 20 adult guinea pigs before the study. Lesion A was injected with 0.1mL 50μg/L bFGF solution once every other day for four times in each guinea pig. The same doses of 0.9% NS were injected in the lesion C as negative control group. And lesion B remained unmanipulated as blank control group. Biopsies from the A,B and C lesions were conducted in the day 21 after injections to make pathological slides. The percentages of the proliferation fibroblasts were calculated and analyzed under microscopy after immunohistochemical stain using mouse anti-human ki-67 antigen monoclonal antibody. Results The average percentage of proliferative fibroblasts in experimental group,negative control group and blank control group were (7.63±1.42)%,(0.98±0.33)% and (1.22±0.34)%,respectively.The average percentage of proliferative fibroblasts in experimental group was obviously higher than that in the control groups (P<0.05). Conclusion Basic fibroblast growth factor can obviously stimulate the proliferation of fibroblasts in the guinea pigs’ cutaneous atrophic scars.