HPLC法测定中药了哥王中伞形花内酯的含量

目的建立测定了哥王中伞形花内酯(umbelliferone)含量的HPLC法。方法色谱柱:伊利特-C18柱(200 mm×4.6 mm,5μm),流动相:甲醇-水-四氢呋喃-冰乙酸(体积比为20.0∶75.0∶5.0∶0.6),检测波长:324 nm,柱温:40℃,流速:1.0 mL.min-1。结果伞形花内酯质量浓度在0.6~24.0mg.L-1内线性关系良好(r=0.9998)。伞形花内酯的平均回收率为98.5%,RSD为1.9%。结论该方法适用于了哥王药材的质量控制。

Determination of the content of umbelliferone in Wikstroemia indica(L.)C.A.Mey by HPLC

Objective To determine the content of umbelliferone in Wikstroemia indica(L.)C. A.Mey. Method An assay method was developed for the determination of the content of the umbelliferone in the Wikstroemia indica(L.)C.A.Mey. It was separated on an Elite C₁₈ODS (200 mm×4.6 mm, 5 μm) column with the mobile phase consisting of a mixture of methanol-water- THF-acetic acid (V:V:V:V=20∶75∶5∶0.6), and UV detection wavelength was set at 324 nm, at the flow rate of 1.0 mL·min^-1, and the column temperature was set at 40 ℃. Results The linear ranges was 0.6-24.0 mg·L^-1(r=0.999 8) for umbelliferone. The average recovery was 98.5% with RSD of 1.9％ ．Conclusions The method is accurate, sensitive with good reproducibility and could be used for the determination of the content of umbelliferone in Wikstroemia indica(L.)C.A.Mey..